RELA/NFκB p65 Antibody (F-6): sc-8008

NFκB p65 Antibody (F-6) is a mouse monoclonal IgG1 kappa light chain antibody raised against amino acids 1-286 at the N-terminus of the NFκB p65 protein of human origin. NFκB p65 monoclonal antibody (F-6) reacts with mouse, rat, and human samples, making NFκB p65 (F-6) antibody ideal for applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). NFκB p65, also known as RelA, is a critical subunit of the NFκB transcription factor complex, which regulates genes involved in inflammation, immune response, cell survival, and proliferation. Activation of NFκB p65 begins with the degradation of IκB proteins, which sequester NFκB in the cytoplasm under resting conditions. Once IκB is degraded, NFκB p65 is released and rapidly translocates to the nucleus, a process facilitated by nuclear localization signals (NLS). This translocation enables NFκB p65 to access and bind specific κB enhancer sequences in the promoter regions of target genes, initiating transcription. Dynamic movement of NFκB p65 between the cytoplasm and nucleus is tightly regulated and crucial for a rapid and context-specific transcriptional response to environmental and cellular signals. Studying this translocation is vital for understanding how dysregulation of NFκB activity contributes to inflammatory diseases and cancer. NFκB p65 monoclonal antibody (F-6) is available in both non-conjugated and conjugated forms, including HRP, PE, FITC, and Alexa Fluor® conjugates.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

RELA/NFκB p65 Antibody (F-6) References:

1. The ankyrin repeat domains of the NF-kappa B precursor p105 and the protooncogene bcl-3 act as specific inhibitors of NF-kappa B DNA binding.  |  Hatada, EN., et al. 1992. Proc Natl Acad Sci U S A. 89: 2489-93. PMID: 1532257
2. Distinct combinations of NF-kappa B subunits determine the specificity of transcriptional activation.  |  Perkins, ND., et al. 1992. Proc Natl Acad Sci U S A. 89: 1529-33. PMID: 1542644
3. The 65-kDa subunit of human NF-kappa B functions as a potent transcriptional activator and a target for v-Rel-mediated repression.  |  Ballard, DW., et al. 1992. Proc Natl Acad Sci U S A. 89: 1875-9. PMID: 1542686
4. Cloning, expression and characterization of the bovine p65 subunit of NFkappaB.  |  Doleschall, M., et al. 2007. Dev Comp Immunol. 31: 945-61. PMID: 17306370
5. Hypertension aggravates glomerular dysfunction with oxidative stress in a rat model of diabetic nephropathy.  |  Tomohiro, T., et al. 2007. Life Sci. 80: 1364-72. PMID: 17331548
6. Glucocorticoid receptor, nuclear factor kappaB, activator protein-1 and C-jun N-terminal kinase in systemic lupus erythematosus patients.  |  Oikonomidou, O., et al. 2006. Neuroimmunomodulation. 13: 194-204. PMID: 17347585
7. Dominant-negative activator protein 1 (TAM67) targets cyclooxygenase-2 and osteopontin under conditions in which it specifically inhibits tumorigenesis.  |  Matthews, CP., et al. 2007. Cancer Res. 67: 2430-8. PMID: 17363560
8. Cloning of an NF-kappa B subunit which stimulates HIV transcription in synergy with p65.  |  Schmid, RM., et al. 1991. Nature. 352: 733-6. PMID: 1876189
9. Cloning of the DNA-binding subunit of human nuclear factor kappa B: the level of its mRNA is strongly regulated by phorbol ester or tumor necrosis factor alpha.  |  Meyer, R., et al. 1991. Proc Natl Acad Sci U S A. 88: 966-70. PMID: 1992489
10. CircRNF10-DHX15 interaction suppressed breast cancer progression by antagonizing DHX15-NF-κB p65 positive feedback loop.  |  Zheng, W., et al. 2023. Cell Mol Biol Lett. 28: 34. PMID: 37101128
11. Tanshinone IIA inhibits cardiomyocyte pyroptosis through TLR4/NF-κB p65 pathway after acute myocardial infarction.  |  Chai, R., et al. 2023. Front Cell Dev Biol. 11: 1252942. PMID: 37766966
12. Suppression of NF-κB signaling by Pseudorabies virus DNA polymerase processivity factor UL42 via recruiting SOCS1 to promote the ubiquitination degradation of p65.  |  Bo, Z., et al. 2023. Vet Microbiol. 287: 109896. PMID: 37931575

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RELA/NFκB p65 Antibody (F-6) has 2,037 citations in a variety of scientific publications.

Primary antibodies like Anti-RELA/NFκB p65 Antibody (F-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RELA/NFκB p65 Antibody (F-6) Specifications

Alternate Product Name: RelA

Product Clone Name: F-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-286

Epitope Target: NFκB p65

Epitope Species: human

Product Reactivity/Detection: NFκB p65

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2,037 citations in various scientific publications

Available Conjugates/Forms for RELA/NFκB p65 Antibody (F-6): AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, TRITC: Rhodamine, X: TransCruz Reagent