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- goat polyclonal IgG, 200 µg/ml; also available as rabbit IgG, 200 µg/ml, sc-7998-R
- epitope corresponding to a short amino acid sequence containing phosphorylated Ser 112 of Bad of mouse origin
- recommended for detection of Ser 112 phosphorylated Bad of mouse origin, Ser 113 phosphorylated Bad of rat origin, and Ser 75 phosphorylated Bad of human origin by WB, IP, IF and ELISA
- blocking peptide, sc-7998 P
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Conditions GénéralesRéférences bibliographiques
Accessoires recommandés:
(Cliquez sur l'application voulue)
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| Espèce |
Nom du Gène |
Gene ID |
Localisation chromosomique |
Numéro d'accession de l'isoforme (ARNm) |
Numéro d'accession de la protéine |
Numéro OMIM™ |
| Humain |
BAD |
572 |
11q13.1 |
NM_004322, NM_032989 |
Q92934
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603167 |
| Souris |
Bad |
12015 |
19 A |
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Q61337
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Non disponible |
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Bad Background Information
Phosphorylation of BAD, a pro-apoptotic member of the Bcl-2 protein family, on either serine 112 or serine 136 is thought to be necessary and sufficient for growth factors to promote cell survival (1–3). Serine 155 is a major site of phosphorylation by protein kinase A (PKA) and serum-induced kinases (1). Serine 155 phosphorylation requires the prior phosphorylation of serine 136, which recruits 14-3-3 proteins that then function to increase the accessibility of serine 155 to survival-promoting kinases (4). Like serine 112 and serine 136, phosphorylation of serine 155 inhibits the pro-apoptotic function of BAD (1). Serine 155 phosphorylation disrupts the binding of BAD to prosurvival Bcl-2 proteins and thereby promotes cell survival (4,5).
| p-Bad (Ser 112) Références bibliographiques |
Consulter les citations pour ces anticorps p-Bad (Ser 112): sc-7998 et/ou un anticorps p-Bad (Ser 112) conjugué.
8 total citations En chargeant des citations.
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p-Bad (Ser 112)
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p-Bad (Ser 112)-R: sc-7998-R. Western blot analysis of phosphorylated Bad expression in HeLa (A) and calyculin-treated HeLa (B) whole cell lysates.
p-Bad (Ser 112)-R: sc-7998-R. Western blot analysis of Bad phosphorylation in non-transfected: sc-117752 (A) and human Bad transfected: sc-170552 (B) 293T whole cell lysates. p-Bad (Ser 112)-R: sc-7998-R. Western blot analysis of Bad phosphorylation in non-transfected: sc-117752 (A), untreated human Bad transfected: sc-170552 (B) and lambda protein phosphatase treated human Bad transfected: sc-170552 (C) 293T whole cell lysates. Western blot analysis of Bad phosphorylation in non-transfected: sc-117752 (A, D), untreated human Bad transfected: sc-170552 (B, E) and lambda protein phosphatase treated human Bad transfected: sc-170552 (C, F) 293T whole cell lysates. Antibodies tested include p-Bad (Ser 112)-R: sc-7998-R (A, B, C) and Bad (H-168): sc-7869 (D, E, F). Western blot analysis of Bad phosphorylation in non-transfected: sc-117752 (A,E), untreated human Bad transfected: sc-170552 (B,F), PMA treated human Bad transfected: sc-170552 (C,G) and PMA and lambda protein phosphatase ( sc-200312A) treated human Bad transfected: sc-170552 (D,H) 293T whole cell lysates.Antibodies tested include p-Bad (Ser 112)-R: sc-7998-R (A,B,C,D) and Bad (H-168): sc-7869 (E,F,G,H). |
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