Annexin V Antibody (H-3): sc-74438

Annexin V Antibody (H-3) is a mouse monoclonal IgG2a kappa light chain antibody designed to detect Annexin V, also known as Lipocortin V or Annexin 5, across multiple platforms including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Annexin V monoclonal antibody (H-3) is raised against amino acids 1-319, representing the full-length human Annexin V, and demonstrates robust reactivity with Annexin V from mouse, rat, and human origins. Annexin V is a critical member of the annexin family of calcium-binding proteins, primarily localized to the plasma membrane where it plays a vital role in the regulation of membrane dynamics and apoptotic cell recognition by binding to phosphatidylserine in a calcium-dependent manner. This localization is essential for processes such as apoptosis, inflammation, and cellular adhesion, making Annexin V a valuable marker in studies of cell death and disease mechanisms. Annexin V monoclonal antibody (H-3) is available in both non-conjugated form and a variety of conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatile options for diverse experimental applications. Anti-Annexin V antibody (H-3) facilitates advanced research into Annexin V′s biological functions and involvement in pathological states. Annexin V monoclonal antibody (H-3) enables researchers to effectively investigate the spatial and temporal expression patterns of Annexin V, thereby gaining deeper insights into its pivotal roles within the cell.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Annexin V Antibody (H-3) References:

1. Radiolabeled annexin V imaging: a useful technique for determining apoptosis in multiple sclerosis.  |  Assadi, M., et al. 2011. Med Hypotheses. 77: 43-6. PMID: 21440374
2. Inhibitory effect of annexin V on protein kinase C activity in mesangial cell lysates.  |  Rothhut, B., et al. 1995. Eur J Biochem. 232: 865-72. PMID: 7588728
3. Annexin IV inhibits calmodulin-dependent protein kinase II-activated chloride conductance. A novel mechanism for ion channel regulation.  |  Chan, HC., et al. 1994. J Biol Chem. 269: 32464-8. PMID: 7798247
4. Differential tissue expression of Annexin VIII in human.  |  Reutelingsperger, CP., et al. 1994. FEBS Lett. 349: 120-4. PMID: 8045287
5. Expression of the annexin VIII gene in acute promyelocytic leukemia.  |  Liu, JH., et al. 1994. Leuk Lymphoma. 13: 381-6. PMID: 8069182
6. Structural evolution of the annexin supergene family.  |  Smith, PD. and Moss, SE. 1994. Trends Genet. 10: 241-6. PMID: 8091504
7. Calcium-dependent binding of S100C to the N-terminal domain of annexin I.  |  Mailliard, WS., et al. 1996. J Biol Chem. 271: 719-25. PMID: 8557678
8. Structural and functional characterisation of the voltage sensor in the ion channel human annexin V.  |  Liemann, S., et al. 1996. J Mol Biol. 258: 555-61. PMID: 8636991
9. The high-resolution crystal structure of human annexin III shows subtle differences with annexin V.  |  Favier-Perron, B., et al. 1996. Biochemistry. 35: 1740-4. PMID: 8639653
10. Mouse annexin V chromosomal localization, cDNA sequence conservation, and molecular evolution.  |  Rodriguez-Garcia, MI., et al. 1996. Genomics. 31: 151-7. PMID: 8824796
11. Annexin V-affinity assay: a review on an apoptosis detection system based on phosphatidylserine exposure.  |  van Engeland, M., et al. 1998. Cytometry. 31: 1-9. PMID: 9450519
12. Mouse annexin V genomic organization includes an endogenous retrovirus.  |  Rodriguez-Garcia, MI., et al. 1999. Biochem J. 337 (Pt 1): 125-31. PMID: 9854034

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Annexin V Antibody (H-3) has 33 citations in a variety of scientific publications.

Primary antibodies like Anti-Annexin V Antibody (H-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Annexin V Antibody (H-3) Specifications

Alternate Product Name: Annexin V

Product Clone Name: H-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-319

Epitope Target: Annexin V

Epitope Species: human

Product Reactivity/Detection: Annexin V

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 33 citations in various scientific publications

Available Conjugates/Forms for Annexin V Antibody (H-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin