epitope corresponding to amino acids 7700-7968 mapping at the C-terminus of Obscurin of human origin
recommended for detection of Obscurin of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including canine and porcine
Obscurin Background Information Belonging to the protein kinase superfamily, Obscurin is a 7968 amino acid protein that is specifically expressed in skeletal and cardiac muscle. Containing a GTPase nucleotide exchange factor (GEF) domain, Obscurin is localized in the sarcomere near the M-band and Z-disk where it participates in the G-protein regulated pathways that control the formation of new myofibrils. Obscurin contains 55 immunoglobulin (Ig)-like domains, through which it interacts with proteins such as titin and calmodulin. During cardiac hypertrophy, the gene encoding Obscurin is upregulated to produce additional contractile units. Knockdown of Obscurin mRNA results in the disruption of M-bands and A-bands and reduction of myosin and myomesin levels, suggesting that Obscurin is required for regular sarcomere structure. There are 6 isoforms of Obscurin that are produced as a result of alternative splicing events.
