p-beta-catenin Antibody (BC-22): sc-57535

p-β-catenin Antibody (BC-22) is a mouse monoclonal IgG2b kappa light chain antibody that detects p-β-catenin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). anti-p-β-catenin antibody (BC-22) is available as a non-conjugated format. p-β-catenin protein, also known as beta-Catenin or CTNNB1, plays a pivotal role in the Wnt signaling pathway, which regulates cell proliferation, differentiation, and migration. p-β-catenin protein maintains cellular adhesion and tissue integrity by interacting with E-cadherin to form catenin/cadherin complexes that mediate cell-cell adhesion. Dysregulation of p-β-catenin protein can lead to aberrant signaling and has been implicated in various cancers, including colorectal and breast cancer. p-β-catenin protein localizes primarily in the cytoplasm and nucleus, where p-β-catenin protein acts as a transcriptional co-activator for Wnt target genes. Nuclear translocation of p-β-catenin protein is tightly regulated, and nuclear accumulation can result from post-translational modifications, such as phosphorylation by glycogen synthase kinase-3β. These modifications influence p-β-catenin protein stability and interactions with other proteins, including tumor suppressor APC and regulator Pin1, which can further modulate signaling pathways. Understanding p-β-catenin protein localization and function is vital for elucidating roles in oncogenesis and developing targeted therapies.

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p-beta-catenin Antibody (BC-22) References:

1. Mutational analysis of the CTNNB1 (beta-catenin) gene in human endometrial cancer: frequent mutations at codon 34 that cause nuclear accumulation.  |  Ikeda, T., et al. 2000. Oncol Rep. 7: 323-6. PMID: 10671680
2. Pin1 regulates turnover and subcellular localization of beta-catenin by inhibiting its interaction with APC.  |  Ryo, A., et al. 2001. Nat Cell Biol. 3: 793-801. PMID: 11533658
3. Regulation of S33/S37 phosphorylated beta-catenin in normal and transformed cells.  |  Sadot, E., et al. 2002. J Cell Sci. 115: 2771-80. PMID: 12077367
4. CK2 phosphorylation of the armadillo repeat region of beta-catenin potentiates Wnt signaling.  |  Song, DH., et al. 2003. J Biol Chem. 278: 24018-25. PMID: 12700239
5. Independent interactions of phosphorylated β-catenin with E-cadherin at cell-cell contacts and APC at cell protrusions.  |  Faux, MC., et al. 2010. PLoS One. 5: e14127. PMID: 21152425
6. Selective 14-3-3γ induction quenches p-β-catenin Ser37/Bax-enhanced cell death in cerebral cortical neurons during ischemia.  |  Lai, XJ., et al. 2014. Cell Death Dis. 5: e1184. PMID: 24743739
7. Phospholipase C-γ1 potentially facilitates subcellular localization of activated β-catenin, p-β-catenin(S552), during bovine herpesvirus 1 productive infection in MDBK cells.  |  Liu, C., et al. 2023. Vet Microbiol. 276: 109626. PMID: 36502739
8. Frequent alterations in E-cadherin and alpha- and beta-catenin expression in human breast cancer cell lines.  |  Pierceall, WE., et al. 1995. Oncogene. 11: 1319-26. PMID: 7478552
9. Role of the E-cadherin/alpha-catenin complex in modulating cell-cell and cell-matrix adhesive properties of invasive colon carcinoma cells.  |  Breen, E., et al. 1995. Ann Surg Oncol. 2: 378-85. PMID: 7496831
10. Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin.  |  Knudsen, KA., et al. 1995. J Cell Biol. 130: 67-77. PMID: 7790378

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-beta-catenin Antibody (BC-22) has 31 citations in a variety of scientific publications.

Primary antibodies like Anti-p-beta-catenin Antibody (BC-22) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-beta-catenin Antibody (BC-22) Specifications

Alternate Product Name: β-catenin

Product Clone Name: BC-22

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: Ser 33/37

Epitope Target: β-catenin

Epitope Species: human

Product Reactivity/Detection: Ser 33/Ser 37 phosphorylated β-catenin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 50 µg IgG_2b kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 31 citations in various scientific publications

Available Conjugates/Forms for p-beta-catenin Antibody (BC-22): N/A

The preferred beta-catenin antibody is beta-catenin Antibody (E-5): sc-7963.