epitope mapping within an internal region of WDR5 of human origin
recommended for detection of WDR5 and WDR5B of mouse and human origin by WB, IF and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
WDR5/5B Background Information WD-repeat protein 5 (WDR5, also designated BMP-2-induced gene 3 kb or BIG-3) belongs to the family of WD-40 repeat proteins, and is essential for vertebrate development, Hox gene activation and global H3K4 trimethylation. WDR5 is a conserved subunit of Trithorax (TRX) histone methyltransferase complexes that selectively binds to dimethylated Lys4 (K4me2) in histone H3 to promote K4 trimethylation by TRX. It is expressed in osteoblasts, chondrocytes, osteocytes and marrow stromal cells. WDR5B (WD repeat domain 5B) is a 330 amino acid member of the WD repeat family. WDR5B is a probable substrate receptor subunity of a CUL-RING E3 protein ligase complex, and has been found to interact with CUL-4B and DDB1. Both WDR5 and WDR5B contain 7 WD-repeats.
