Date published: 2026-8-14
访问圣克鲁斯动物健康

1-800-457-3801

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
cathepsin L Antibody (33/2) - Immunohistochemistry - Image 12100
cathepsin L 抗体 (33/2): sc-32320. 福尔马林固定, 石蜡包埋的人类乳腺肿瘤的免疫过氧化物酶染色, 显示细胞质定位.
cathepsin L 抗体 (33/2): sc-32320. 福尔马林固定, 石蜡包埋的人类乳腺肿瘤的免疫过氧化物酶染色, 显示细胞质定位.

cathepsin L 抗体 (33/2): sc-32320

3.9(11)
寫評論提問

说明书
  • cathepsin L 抗体 33/2 是小鼠单克隆 IgG1 κ，cathepsin L抗体, 在21篇文献中引用，规格为200 µg/ml
  • 针对human原cathepsin L
  • cathepsin L 抗体 (33/2) 推荐用于WB, IP, IF 和 IHC(P)，检测human，以及在较小程度上，检测mouse 和 rat来源的cathepsin L and procathepsin L
  • 抗cathepsin L抗体（33/2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP和 m-IgG1 BP-HRP是cathepsin L Antibody (33/2) for WB and IHC(P) applications. 的首选辅助检测试剂，这些试剂现与cathepsin L Antibody (33/2) 打包提供（请参阅下面的订购信息）。
Gene Editing Promo Banner

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Cathepsin抗体，包括cathepsin 6, cathepsin A, cathepsin B, cathepsin C, cathepsin D, cathepsin E, cathepsin H, cathepsin K, cathepsin L, cathepsin O, cathepsin S, cathepsin V, cathepsin W, cathepsin Z和pan cathepsin

Cathepsin L 抗体（33/2）是一种小鼠单克隆 IgG1 kappa 轻链抗体，用于检测 cathepsin L 蛋白，也称为 CTSL、主要排泄蛋白（MEP）或 CATL。抗组织蛋白酶 L 抗体 (33/2) 针对人类前组织蛋白酶 L 产生，在人类样本中与组织蛋白酶 L 及其前酶形式均表现出强烈的反应性，并与小鼠和大鼠物种存在交叉反应，因此非常适合用于蛋白质印迹 (WB)、免疫沉淀 (IP)、免疫荧光 (IF) 和石蜡包埋切片免疫组织化学 (IHCP)。Cathepsin L是一种重要的溶酶体半胱氨酸蛋白酶，参与胶原蛋白、弹性蛋白和α-1蛋白酶抑制剂等蛋白质的细胞内降解，在细胞代谢和蛋白质分解代谢中发挥着重要作用。这种蛋白酶对于维持细胞内环境至关重要，并参与多种生理过程，包括组织重塑和免疫反应。 组织蛋白酶 L (33/2) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和各种 Alexa Fluor® 结合物，为不同的检测方法提供了灵活性。作为蛋白酶C1家族的一员，组织蛋白酶L以二聚体形式存在，由来自单个前体蛋白的二硫键连接的重链和轻链组成，从而确保研究中可靠且一致的性能。无论是研究蛋白质降解途径、细胞代谢还是蛋白酶在疾病中的作用，组织蛋白酶L单克隆抗体（33/2）都能提供推进科学发现所需的特异性和多功能性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

cathepsin L 抗体 (33/2) 参考文献:

  1. 骨骼发育过程中胰蛋白酶的表达  |  Söderström, M., et al. 1999. Biochim Biophys Acta. 1446: 35-46. PMID: 10395917
  2. 人 cathepsin L 启动子的克隆和表征。  |  Bakhshi, R., et al. 2001. Gene. 275: 93-101. PMID: 11574156
  3. 人类 cathepsin L mRNA 的剪接变体显示出不同的表达率。  |  Abudula, A., et al. 2001. Biol Chem. 382: 1583-91. PMID: 11767948
  4. 新型人类凝血酶 L 剪接变体的鉴定和特征描述。  |  Arora, S. and Chauhan, SS. 2002. Gene. 293: 123-31. PMID: 12137950
  5. 鉴定白细胞介素-8 转换酶为 cathepsin L。  |  Ohashi, K., et al. 2003. Biochim Biophys Acta. 1649: 30-9. PMID: 12818188
  6. 手术肺癌中 cathepsin B 和 cathepsin L 的表达, 增殖活性和临床意义。  |  Kayser, K., et al. 2003. Anticancer Res. 23: 2767-72. PMID: 12926111
  7. 骨骼肌中猫蛋白酶 L 基因表达受损与 2 型糖尿病有关。  |  Huang, X., et al. 2003. Diabetes. 52: 2411-8. PMID: 12941783
  8. 组织蛋白酶L在癌症治疗中的靶向作用。  |  Sudhan, DR. and Siemann, DW. 2015. Pharmacol Ther. 155: 105-16. PMID: 26299995
  9. 人和小鼠前凝血酶 L 的完整核苷酸和推导氨基酸序列。  |  Joseph, LJ., et al. 1988. J Clin Invest. 81: 1621-9. PMID: 2835398
  10. 组织蛋白酶L在新型冠状病毒肺炎中的研究：从药理学证据到遗传学。  |  Gomes, CP., et al. 2020. Front Cell Infect Microbiol. 10: 589505. PMID: 33364201
  11. 大鼠 cathepsin L 的 cDNA 分子克隆和测序。  |  Ishidoh, K., et al. 1987. FEBS Lett. 223: 69-73. PMID: 3666143
  12. 嗜酸性粒细胞通过组织蛋白酶L促进肺基质破坏和肺气肿。  |  Xu, X., et al. 2023. Signal Transduct Target Ther. 8: 390. PMID: 37816708

订购信息

产品名称产品编号规格价格数量收藏夹

cathepsin L 抗体 (33/2)

sc-32320
200 µg/ml
$322.00

cathepsin L (33/2): m-IgG Fc BP-HRP 套装

sc-526678
200 µg Ab; 10 µg BP
$361.00

cathepsin L (33/2): m-IgG1 BP-HRP 套装

sc-532051
200 µg Ab; 20 µg BP
$361.00

cathepsin L 抗体 (33/2) AC

sc-32320 AC
500 µg/ml, 25% agarose
$424.00

cathepsin L 抗体 (33/2) HRP

sc-32320 HRP
200 µg/ml
$322.00

cathepsin L 抗体 (33/2) FITC

sc-32320 FITC
200 µg/ml
$336.00

cathepsin L 抗体 (33/2) PE

sc-32320 PE
200 µg/ml
$349.00

cathepsin L 抗体 (33/2) Alexa Fluor® 488

sc-32320 AF488
200 µg/ml
$364.00

cathepsin L 抗体 (33/2) Alexa Fluor® 546

sc-32320 AF546
200 µg/ml
$364.00

cathepsin L 抗体 (33/2) Alexa Fluor® 594

sc-32320 AF594
200 µg/ml
$364.00

cathepsin L 抗体 (33/2) Alexa Fluor® 647

sc-32320 AF647
200 µg/ml
$364.00

cathepsin L 抗体 (33/2) Alexa Fluor® 680

sc-32320 AF680
200 µg/ml
$364.00

cathepsin L 抗体 (33/2) Alexa Fluor® 790

sc-32320 AF790
200 µg/ml
$364.00

What is the recommended titer to use when staining on fixed cells

Asked by: Paige
Thank you for your question.We recommend a starting dilution of 1:50-1:500.
Answered by: BlakeJ
Date published: 2024-09-03

I am using cathepsin L (33/2): sc-32320 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Germaine
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
  • y_2026, m_8, d_14, h_4CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.48
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_zh_CN, sid_32320, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 107ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from Acceptable Performance for Research ApplicationsThe anti-CTSL antibody provided acceptable performance in our experiments. It allowed us to detect the target protein with satisfactory results, although some optimization was required to obtain the best signal. Overall, it was suitable for our research and met our experimental needs
Date published: 2026-08-07
Rated 5 out of 5 by from Very goodThe Ab was tested for WB, IF and IHC, worked well.
Date published: 2021-11-29
Rated 5 out of 5 by from Excellent IF staining in VeroE6 cellsGreat staining in VeroE6 cells (1:200, methanol fixation) by IF
Date published: 2021-05-04
Rated 4 out of 5 by from good antibodyThis antibody delivered very specific location and moderate signal
Date published: 2018-02-14
Rated 3 out of 5 by from WBThis antibody worked ok for me with 1:200 and ECL prime
Date published: 2017-05-30
Rated 1 out of 5 by from keine Ergebnisse in IHCVor ein paar Wochen Testmenge bestellt. Antikörper hat überhaupt nicht funktioniert. aber Kontrollslides schon. Leider nur Zeitverschwendung.
Date published: 2017-03-08
Rated 5 out of 5 by from Well rounded antibodyGreat antibody all around, really strong for Western blot, IHC and for IF. Smart pick!
Date published: 2017-02-08
Rated 4 out of 5 by from Worked well for immunohistochemical investigationWorked well for immunohistochemical investigation - used 1:1000 dilution on fomalin fixed paraffin embedded temporal lobe sections (human). -SCBT Publication Review
Date published: 2015-05-17
  • y_2026, m_8, d_14, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.48
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_12
  • loc_zh_CN, sid_32320, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 9ms
  • REVIEWS, PRODUCT
cathepsin L抗体 (33/2) is rated 3.9 out of 5 by 12.
  • y_2026, m_8, d_14, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.48
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_12
  • loc_zh_CN, sid_32320, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 125ms
  • REVIEWS, PRODUCT
1-800-457-3801
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打1-800-457-3801联系我们。
版权所有© 2007-2026 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。