p-Akt1/2/3 Antibody (B-5): sc-271966

p-Akt1/2/3 Antibody (B-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects p-Akt1/2/3, specifically Thr 308 phosphorylated Akt1, Thr 309 phosphorylated Akt2, and Thr 305 phosphorylated Akt3, in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). p-Akt1/2/3 (B-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The Akt family of serine/threonine kinases, which includes Akt1, Akt2, and Akt3, plays a crucial role in cellular signaling pathways that regulate metabolism, growth, and survival. Notably, Akt proteins are activated by insulin and insulin-like growth factor-1 (IGF-1), leading to phosphorylation at specific threonine and serine residues, which is essential for their function in promoting glucose uptake and glycogen synthesis. The pleckstrin homology domain present in all Akt family members is vital for their membrane localization and subsequent activation by phosphatidylinositol 3-kinase (PI3K) signaling. Akt3, while also responsive to insulin, has unique regulatory mechanisms that differentiate its activation from that of Akt1 and Akt2, particularly in the context of protein kinase C signaling. Understanding the intricate roles of these proteins in various biological processes, including cellular proliferation and apoptosis, underscores the importance of anti-p-Akt1/2/3 antibody (B-5) in research focused on metabolic diseases, cancer, and other conditions where Akt signaling is disrupted.

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p-Akt1/2/3 Antibody (B-5) References:

1. Cytoplasmic Skp2 expression is associated with p-Akt1 and predicts poor prognosis in human breast carcinomas.  |  Liu, J., et al. 2012. PLoS One. 7: e52675. PMID: 23300741
2. Discovery of 4-(Piperazin-1-yl)-7H-pyrrolo[2,3-d]pyrimidine Derivatives as Akt Inhibitors.  |  Liu, Y., et al. 2016. Arch Pharm (Weinheim). 349: 356-62. PMID: 26991997
3. Slug inhibits the proliferation and tumor formation of human cervical cancer cells by up-regulating the p21/p27 proteins and down-regulating the activity of the Wnt/β-catenin signaling pathway via the trans-suppression Akt1/p-Akt1 expression.  |  Cui, N., et al. 2016. Oncotarget. 7: 26152-67. PMID: 27036045
4. Quercetin Inhibits the Migration and Invasion of HCCLM3 Cells by Suppressing the Expression of p-Akt1, Matrix Metalloproteinase (MMP) MMP-2, and MMP-9.  |  Lu, J., et al. 2018. Med Sci Monit. 24: 2583-2589. PMID: 29701200
5. P-AKT2/SPK1 (P-SPK1) and P-MEK/P-ERK cell signaling pathways are involved in LPS-induced macrophage migration.  |  Lei, Y., et al. 2019. Am J Transl Res. 11: 2725-2741. PMID: 31217849
6. Expression of Akt1 and p-Akt1 in peripheral T cell subsets of multiple sclerosis patients.  |  Oktelik, FB., et al. 2021. Acta Neurol Belg. 121: 1777-1782. PMID: 33034831
7. Hexokinases 2 promoted cell motility and distant metastasis by elevating fibronectin through Akt1/p-Akt1 in cervical cancer cells.  |  Chen, Q., et al. 2021. Cancer Cell Int. 21: 600. PMID: 34758823
8. Hexokinase 2 promoted cell motility and proliferation by activating Akt1/p-Akt1 in human ovarian cancer cells.  |  Tian, X., et al. 2022. J Ovarian Res. 15: 92. PMID: 35953860
9. The Significance of p-AKT1 as a Prognostic Marker and Therapeutic Target in Patients With Hormone Receptor-Positive and Human Epidermal Growth Factor Receptor-2-Positive Early Breast Cancer.  |  Kim, JY., et al. 2022. J Breast Cancer. 25: 387-403. PMID: 36314765
10. IL-21/23 axis modulates inflammatory cytokines and RANKL expression in RA CD4+ T cells via p-Akt1 signaling.  |  Bhattacharya, G., et al. 2023. Front Immunol. 14: 1235514. PMID: 37809066

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Akt1/2/3 Antibody (B-5) has 114 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Akt1/2/3 Antibody (B-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Akt1/2/3 Antibody (B-5) Specifications

Alternate Product Name: Akt1/2/3

Product Clone Name: B-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: Thr 308/309/305

Epitope Target: Akt2

Epitope Species: human

Product Reactivity/Detection: Thr 308 phosphorylated Akt1 and correspondingly Thr 309 phosphorylated Akt2 and correspondingly Thr 305 phosphorylated Akt3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 114 citations in various scientific publications

Available Conjugates/Forms for p-Akt1/2/3 Antibody (B-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Akt1 antibody is Akt1 Antibody (B-1): sc-5298.