Topo IIβ Antibody (H-8): sc-25330

Topo IIβ Antibody (H-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Topo II beta protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Topo II beta antibody (H-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Topo II beta protein plays a crucial role in DNA replication and transcription by introducing transient double-strand breaks in DNA, allowing for relaxation of supercoiled DNA, which is essential for proper chromosomal segregation during cell division. This function is particularly important in rapidly dividing cells, where DNA topology regulation is vital for maintaining genomic stability. Differential expression of Topo II isoforms, including Topo IIα and Topo IIβ, suggests distinct roles in cellular processes, with Topo IIβ being implicated in specific tissue functions and responses to DNA damage. Understanding Topo IIβ′s unique contributions can provide insights into potential therapeutic targeting in cancer treatment, where topoisomerase inhibitors are commonly used to disrupt cancer cell proliferation.

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Topo IIβ Antibody (H-8) References:

1. Topoisomerase II alpha and topoisomerase II beta genes: characterization and mapping to human chromosomes 17 and 3, respectively.  |  Tan, KB., et al. 1992. Cancer Res. 52: 231-4. PMID: 1309226
2. Structure of the human type I DNA topoisomerase gene.  |  Kunze, N., et al. 1991. J Biol Chem. 266: 9610-6. PMID: 1851751
3. Isolation and characterization of a human cDNA clone encoding a novel DNA topoisomerase II homologue from HeLa cells.  |  Austin, CA. and Fisher, LM. 1990. FEBS Lett. 266: 115-7. PMID: 2163884
4. HIV-1 associated Topoisomerase IIβ kinase: a potential pharmacological target for viral replication.  |  Ponraj, K., et al. 2013. Curr Pharm Des. 19: 4776-86. PMID: 23260019
5. Characterization and immunological identification of cDNA clones encoding two human DNA topoisomerase II isozymes.  |  Chung, TD., et al. 1989. Proc Natl Acad Sci U S A. 86: 9431-5. PMID: 2556712
6. cDNA cloning of human DNA topoisomerase I: catalytic activity of a 67.7-kDa carboxyl-terminal fragment.  |  D'Arpa, P., et al. 1988. Proc Natl Acad Sci U S A. 85: 2543-7. PMID: 2833744
7. The Roles of DNA Topoisomerase IIβ in Transcription.  |  Madabhushi, R. 2018. Int J Mol Sci. 19: PMID: 29966298
8. Dynamic behavior of DNA topoisomerase IIβ in response to DNA double-strand breaks.  |  Morotomi-Yano, K., et al. 2018. Sci Rep. 8: 10344. PMID: 29985428
9. Calcineurin dephosphorylates topoisomerase IIβ and regulates the formation of neuronal-activity-induced DNA breaks.  |  Delint-Ramirez, I., et al. 2022. Mol Cell. 82: 3794-3809.e8. PMID: 36206766
10. MeCP2 represses the activity of topoisomerase IIβ in long neuronal genes.  |  Nettles, SA., et al. 2023. Cell Rep. 42: 113538. PMID: 38096051
11. The mechanisms of DNA topoisomerases.  |  Roca, J. 1995. Trends Biochem Sci. 20: 156-60. PMID: 7770916
12. A model for the mechanism of human topoisomerase I.  |  Stewart, L., et al. 1998. Science. 279: 1534-41. PMID: 9488652

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Topo IIβ Antibody (H-8) has 28 citations in a variety of scientific publications.

Primary antibodies like Anti-Topo IIβ Antibody (H-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Topo IIβ Antibody (H-8) Specifications

Alternate Product Name: DNA topoisomerase II beta

Product Clone Name: H-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1341-1626

Epitope Target: Topo IIβ

Epitope Species: human

Product Reactivity/Detection: Topo IIβ

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 28 citations in various scientific publications

Available Conjugates/Forms for Topo IIβ Antibody (H-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin