CD2AP Antibody (B-4): sc-25272

CD2AP Antibody (B-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects CD2AP of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). CD2AP (B-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD2-associated protein (CD2AP) plays a crucial role as a cytosolic adaptor molecule that interacts with the intracellular portion of CD2 during T cell activation, facilitating cell adhesion through CD2-mediated binding to ligands on antigen-presenting cells. This interaction enhances antigen-specific T cell activation, promotes cell clustering, and induces cytoskeletal polarization, which are essential processes in the immune response. CD2AP is predominantly expressed in the liver, thymus, and spleen, and CD2AP structure includes three Src Homology 3 (SH3) domains that are critical for binding to CD2. Disruption of this interaction due to mutations in CD2AP can lead to impaired cell clustering and polarization in activated T lymphocytes, highlighting CD2AP importance in T cell function. Studies have shown that mice lacking CD2AP develop a lethal congenital nephrotic syndrome, underscoring CD2AP role in maintaining the integrity of the renal glomerulus.

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CD2AP Antibody (B-4) References:

1. Congenital nephrotic syndrome in mice lacking CD2-associated protein.  |  Shih, NY., et al. 1999. Science. 286: 312-5. PMID: 10514378
2. The relationship among nephrin, podocin, CD2AP, and alpha-actinin might not be a true 'interaction' in podocyte.  |  Fan, Q., et al. 2006. Kidney Int. 69: 1207-15. PMID: 16501493
3. Autocrine VEGF-A system in podocytes regulates podocin and its interaction with CD2AP.  |  Guan, F., et al. 2006. Am J Physiol Renal Physiol. 291: F422-8. PMID: 16597608
4. Differential interactions between transforming growth factor-beta3/TbetaR1, TAB1, and CD2AP disrupt blood-testis barrier and Sertoli-germ cell adhesion.  |  Xia, W., et al. 2006. J Biol Chem. 281: 16799-813. PMID: 16617054
5. CFBP is a novel tyrosine-phosphorylated protein that might function as a regulator of CIN85/CD2AP.  |  Konishi, H., et al. 2006. J Biol Chem. 281: 28919-31. PMID: 16895919
6. CD2AP/CIN85 balance determines receptor tyrosine kinase signaling response in podocytes.  |  Tossidou, I., et al. 2007. J Biol Chem. 282: 7457-64. PMID: 17213204
7. The role of CD2AP in the Pathogenesis of Alzheimer's Disease.  |  Tao, QQ., et al. 2019. Aging Dis. 10: 901-907. PMID: 31440393
8. CD2AP is a potential prognostic biomarker of renal clear cell carcinoma.  |  Chen, C., et al. 2024. Cancer Med. 13: e7055. PMID: 38457255
9. CD2AP promotes the progression of glioblastoma multiforme via TRIM5-mediated NF-kB signaling.  |  Zhang, L., et al. 2024. Cell Death Dis. 15: 722. PMID: 39353894
10. Making the T cell receptor go the distance: a topological view of T cell activation.  |  Shaw, AS. and Dustin, ML. 1997. Immunity. 6: 361-9. PMID: 9133415
11. A novel adaptor protein orchestrates receptor patterning and cytoskeletal polarity in T-cell contacts.  |  Dustin, ML., et al. 1998. Cell. 94: 667-77. PMID: 9741631
12. Identification of a proline-binding motif regulating CD2-triggered T lymphocyte activation.  |  Nishizawa, K., et al. 1998. Proc Natl Acad Sci U S A. 95: 14897-902. PMID: 9843987

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD2AP Antibody (B-4) has 30 citations in a variety of scientific publications.

Primary antibodies like Anti-CD2AP Antibody (B-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD2AP Antibody (B-4) Specifications

Alternate Product Name: CD2 Associated Protein

Product Clone Name: B-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 350-639

Epitope Target: CD2AP

Epitope Species: human

Product Reactivity/Detection: CD2AP

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 30 citations in various scientific publications

Available Conjugates/Forms for CD2AP Antibody (B-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin