CD16 Antibody (DJ130c): sc-20052
- CD16 Antibody (DJ130c) is a mouse monoclonal IgG1 κ CD16 antibody, cited in 34 publications, provided at 200 µg/ml
- raised against CD16
- CD16 Antibody (DJ130c) is recommended for detection of CD16 of human origin by WB, IP, IF, IHC(P) and FCM
- Anti-CD16 Antibody (DJ130c) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for CD16 Antibody (DJ130c) for WB and IHC(P) applications. These reagents are now offered in bundles with CD16 Antibody (DJ130c) (see ordering information below).
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CD16 Antibody (DJ130c) is a mouse monoclonal IgG1 kappa light chain antibody, designed to detect human CD16, also known as Fc gamma receptor III (FcγRIII), across a variety of applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). CD16 is a crucial immune receptor that exists in two primary isoforms: CD16A (FcγRIIIA), a transmembrane protein expressed on natural killer (NK) cells and macrophages, and CD16B (FcγRIIIB), a glycosylphosphatidylinositol (GPI)-anchored protein found on neutrophils. The distinct structural configurations of these isoforms are essential for their specific roles in the immune response, with CD16A requiring association with the γ subunit of FcεRI or the ζ subunit of the TCR-CD3 complex for proper cell surface expression. This structural versatility allows CD16 to mediate antibody-dependent cellular cytotoxicity (ADCC) and phagocytosis by binding the Fc region of IgG antibodies, thereby targeting and eliminating pathogens or infected cells effectively. CD16′s ability to exist both as a transmembrane and a GPI-anchored receptor highlights versatile functions in immune regulation and response. CD16 monoclonal antibody (DJ130c) is available in both non-conjugated form and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® variants, making CD16 (DJ130c) antibody adaptable for a wide range of experimental applications. Anti-CD16 antibody (DJ130c) is indispensable for research into immune system dynamics, autoimmune disorders, and conditions such as paroxysmal nocturnal hemoglobinuria (PNH), where CD16 expression is significantly altered. With alternate names including FcγRIII, Fc gamma receptor III, CD16A, and CD16B, CD16 (DJ130c) monoclonal antibody provides comprehensive detection capabilities for studying the diverse structural and functional roles of CD16 within the immune system.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CD16 Antibody (DJ130c) References:
- The membrane anchor influences ligand binding two-dimensional kinetic rates and three-dimensional affinity of FcgammaRIII (CD16). | Chesla, SE., et al. 2000. J Biol Chem. 275: 10235-46. PMID: 10744709
- The PI-linked receptor FcRIII is released on stimulation of neutrophils. | Huizinga, TW., et al. 1988. Nature. 333: 667-9. PMID: 2967437
- The Fc receptor for IgG on human natural killer cells: phenotypic, functional, and comparative studies with monoclonal antibodies. | Perussia, B., et al. 1984. J Immunol. 133: 180-9. PMID: 6233371
- Human neutrophil Fc gamma receptor distribution and structure. | Fleit, HB., et al. 1982. Proc Natl Acad Sci U S A. 79: 3275-9. PMID: 6808506
- Ligand binding and phagocytosis by CD16 (Fc gamma receptor III) isoforms. Phagocytic signaling by associated zeta and gamma subunits in Chinese hamster ovary cells. | Nagarajan, S., et al. 1995. J Biol Chem. 270: 25762-70. PMID: 7592758
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD16 Antibody (DJ130c) | sc-20052 | 200 µg/ml | $322.00 | |||
CD16 Antibody (DJ130c): m-IgG Fc BP-HRP Bundle | sc-526594 | 200 µg Ab; 10 µg BP | $361.00 | |||
CD16 Antibody (DJ130c): m-IgG1 BP-HRP Bundle | sc-531967 | 200 µg Ab; 20 µg BP | $361.00 | |||
CD16 Antibody (DJ130c) AC | sc-20052 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CD16 Antibody (DJ130c) HRP | sc-20052 HRP | 200 µg/ml | $322.00 | |||
CD16 Antibody (DJ130c) FITC | sc-20052 FITC | 200 µg/ml | $336.00 | |||
CD16 Antibody (DJ130c) PE | sc-20052 PE | 200 µg/ml | $349.00 | |||
CD16 Antibody (DJ130c) Alexa Fluor® 488 | sc-20052 AF488 | 200 µg/ml | $364.00 | |||
CD16 Antibody (DJ130c) Alexa Fluor® 546 | sc-20052 AF546 | 200 µg/ml | $364.00 | |||
CD16 Antibody (DJ130c) Alexa Fluor® 594 | sc-20052 AF594 | 200 µg/ml | $364.00 | |||
CD16 Antibody (DJ130c) Alexa Fluor® 647 | sc-20052 AF647 | 200 µg/ml | $364.00 | |||
CD16 Antibody (DJ130c) Alexa Fluor® 680 | sc-20052 AF680 | 200 µg/ml | $364.00 | |||
CD16 Antibody (DJ130c) Alexa Fluor® 790 | sc-20052 AF790 | 200 µg/ml | $364.00 |