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- mouse monoclonal IgG2b, 200 µg/ml
- specific for an epitope containing phosphorylated Ser 112 of Bad of mouse origin
- recommended for detection of Ser 112 phosphorylated Bad of mouse, rat and human origin by WB, IP, IF and ELISA
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Conditions Générales
Accessoires recommandés:
(Cliquez sur l'application voulue)
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| Espèce |
Nom du Gène |
Gene ID |
Localisation chromosomique |
Numéro d'accession de l'isoforme (ARNm) |
Numéro d'accession de la protéine |
Numéro OMIM™ |
| Humain |
BAD |
572 |
11q13.1 |
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Q92934
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603167 |
| Souris |
Bad |
12015 |
19 A |
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Q61337
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Non disponible |
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p-Bad Background Information
Phosphorylation of BAD, a pro-apoptotic member of the Bcl-2 protein family, on either serine 112 or serine 136 is thought to be necessary and sufficient for growth factors to promote cell survival (1–3). Serine 155 is a major site of phosphorylation by protein kinase A (PKA) and serum-induced kinases (1). Serine 155 phosphorylation requires the prior phosphorylation of serine 136, which recruits 14-3-3 proteins that then function to increase the accessibility of serine 155 to survival-promoting kinases (4). Like serine 112 and serine 136, phosphorylation of serine 155 inhibits the pro-apoptotic function of BAD (1). Serine 155 phosphorylation disrupts the binding of BAD to prosurvival Bcl-2 proteins and thereby promotes cell survival (4,5). |
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p-Bad (C-10)
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Western blot analysis of Bad phosphorylation in non-transfected: sc-117752 (A,E), untreated human Bad transfected: sc-170552 (B,F), PMA treated human Bad transfected: sc-170552 (C,G) and PMA and lambda protein phosphatase ( sc-200312A) treated human Bad transfected: sc-170552 (D,H) 293T whole cell lysates.Antibodies tested include p-Bad (C-10): sc-166932 (A,B,C,D) and Bad (H-168): sc-7869 (E,F,G,H). Western blot analysis of Bad phosphorylation in non-transfected: sc-117752 (A), untreated human Bad transfected: sc-170552 (B), PMA treated human Bad transfected: sc-170552 (C) and PMA and lambda protein phosphatase treated human Bad transfected: sc-170552 (D) 293T whole cell lysates. Antibodies tested include p-Bad (Ser 112)-R: sc-7998-R (A,B,C,D). |
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