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- mouse monoclonal IgG1, 200µg/ml
- specific for an epitope mapping between amino acids Ser 988 of BRCA1 of human origin
- recommended for detection of Ser 988 phosphorylated BRCA1 of human origin by WB, IP, IF and ELISA
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Conditions Générales
Accessoires recommandés:
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| Espèce |
Nom du Gène |
Gene ID |
Localisation chromosomique |
Numéro d'accession de l'isoforme (ARNm) |
Numéro d'accession de la protéine |
Numéro OMIM™ |
| Humain |
BRCA1 |
672 |
17q21.31 |
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P38398
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113705 |
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p-BRCA1 Background Information
BRCA1 is a cell cycle-regulated nuclear protein that is phosphorylated mainly on serine and to a lesser extent on threonine residues (1). Changes in phosphorylation occur in response to cell cycle progression and DNA damage (1). BRCA1 undergoes hyperphosphorylation during late G1 and S phases of the cell cycle (1). BRCA1 is a substrate of ATM kinase, and phosphorylation of BRCA1 requires the presence of a functional ATM protein (2,3). Chk2 regulates BRCA1 function after DNA damage by phosphorylating Serine 988 of BRCA1 (4). This phosphorylation is required for the release of BRCA1 from Chk2 and the ability of BRCA1 to restore survival after DNA damage (4). BRCA1 is also phosphorylated at Serine 1497, which is part of a cyclin-dependent kinase consensus site (1).
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p-BRCA1 (G-4)
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Western blot analysis of BRCA1 phosphorylation in untreated (A,C) and UV irradiated (B,D) HeLa whole cell lysates. Antibodies tested include p-BRCA1 (G-4): sc-166793 (A,B) and BRCA1 (H-300): sc-28234 (C,D). Western blot analysis of BRCA1 phosphorylation in untreated (A,C) and UV irradiated (B,D) HeLa whole cell lysates. Antibodies tested include p-BRCA1 (G-4): sc-166793 (A,B) and BRCA1 (H-300): sc-28234 (C,D). p-BRCA1 (G-4): sc-166793. Western blot analysis of BRCA1 phosphorylation in untreated (A) and UV irradiated (B) Cos whole cell lysates.
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