Ah Receptor Antibody (A-3): sc-133088

Ah Receptor Antibody (A-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects aryl hydrocarbon receptor protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Aryl hydrocarbon receptor antibody (A-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The aryl hydrocarbon receptor (AhR) plays a crucial role in mediating the biological effects of environmental toxins, such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), which is known to induce adverse reproductive, immunologic, and metabolic effects. As a ligand-dependent transcription factor, AhR is essential for regulating the expression of genes involved in xenobiotic metabolism. Upon binding to TCDD or other ligands, AhR forms a complex with the Ah Receptor nuclear translocator (Arnt), facilitating its translocation to the nucleus where it binds to specific DNA sequences known as xenobiotic responsive elements (XREs). This interaction is vital for the transcriptional activation of genes that help detoxify harmful compounds, underscoring the importance of AhR in maintaining cellular homeostasis and responding to environmental stressors.

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Ah Receptor Antibody (A-3) References:

1. Identification of the Ah receptor nuclear translocator protein (Arnt) as a component of the DNA binding form of the Ah receptor.  |  Reyes, H., et al. 1992. Science. 256: 1193-5. PMID: 1317062
2. Ah receptor-mediated suppression of liver regeneration through NC-XRE-driven p21Cip1 expression.  |  Jackson, DP., et al. 2014. Mol Pharmacol. 85: 533-41. PMID: 24431146
3. Suppression of Ah Receptor (AhR) increases the aggressiveness of TNBC cells and 11-Cl-BBQ-activated AhR inhibits their growth.  |  Elson, DJ., et al. 2023. Biochem Pharmacol. 215: 115706. PMID: 37506922
4. Immune cell-intrinsic Ah receptor facilitates the expression of antimicrobial REG3G in the small intestine.  |  Chakraborty, D., et al. 2024. FASEB J. 38: e23471. PMID: 38358358
5. The Ah receptor: mediator of the toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and related compounds.  |  Okey, AB., et al. 1994. Toxicol Lett. 70: 1-22. PMID: 8310450
6. Functional characterization of DNA-binding domains of the subunits of the heterodimeric aryl hydrocarbon receptor complex imputing novel and canonical basic helix-loop-helix protein-DNA interactions.  |  Bacsi, SG. and Hankinson, O. 1996. J Biol Chem. 271: 8843-50. PMID: 8621524
7. cDNA cloning and tissue-specific expression of a novel basic helix-loop-helix/PAS factor (Arnt2) with close sequence similarity to the aryl hydrocarbon receptor nuclear translocator (Arnt).  |  Hirose, K., et al. 1996. Mol Cell Biol. 16: 1706-13. PMID: 8657146
8. A nuclear localization signal of human aryl hydrocarbon receptor nuclear translocator/hypoxia-inducible factor 1beta is a novel bipartite type recognized by the two components of nuclear pore-targeting complex.  |  Eguchi, H., et al. 1997. J Biol Chem. 272: 17640-7. PMID: 9211913
9. Ah receptor-dependent CYP1A induction by two carotenoids, canthaxanthin and beta-apo-8'-carotenal, with no affinity for the TCDD binding site.  |  Gradelet, S., et al. 1997. Biochem Pharmacol. 54: 307-15. PMID: 9271336
10. Molecular evolution of two vertebrate aryl hydrocarbon (dioxin) receptors (AHR1 and AHR2) and the PAS family.  |  Hahn, ME., et al. 1997. Proc Natl Acad Sci U S A. 94: 13743-8. PMID: 9391097
11. A mutation in the aryl hydrocarbon receptor (AHR) in a cultured mammalian cell line identifies a novel region of AHR that affects DNA binding.  |  Sun, W., et al. 1997. J Biol Chem. 272: 31845-54. PMID: 9395531
12. Animal models of human response to dioxins.  |  Grassman, JA., et al. 1998. Environ Health Perspect. 106 Suppl 2: 761-75. PMID: 9599728

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ah Receptor Antibody (A-3) has 109 citations in a variety of scientific publications.

Primary antibodies like Anti-Ah Receptor Antibody (A-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ah Receptor Antibody (A-3) Specifications

Alternate Product Name: aryl hydrocarbon receptor

Product Clone Name: A-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 637-848

Epitope Target: Ah Receptor

Epitope Species: human

Product Reactivity/Detection: Ah Receptor

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 109 citations in various scientific publications

Available Conjugates/Forms for Ah Receptor Antibody (A-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent