epitope mapping within an internal region of KBTBD10 of human origin
recommended for detection of KBTBD10 isoforms Long and Short of mouse, rat and human origin by WB, IF and ELISA; non cross-reactive with other KBTBD family members; also reactive with additional species, including equine, canine, bovine, porcine and avian
KBTBD10 Background Information KBTBD10 (Kelch repeat and BTB domain-containing protein 10), also known as Kel-like protein 23, sarcosin or Kelch-related protein 1, is a 606 amino acid cytoplasmic protein found in sarcomeric muscle. KBTBD10 plays an important role in the protein ubiquitination pathway by acting as the substrate-specific adaptor of an E3 ubiquitin-protein ligase complex. KBTBD10 forms this complex with CUL-3 and Rbx1, and also interacts with N-RAP. Although predominantly cytoplasmic, KBTBD10 can co-localize with Actin at the ruffle-like membrane structures located at the tips of pseudopodia, indicating a role in pseudopod elongation in transformed cells. KBTBD10 contains five Kelch repeats and one BTB (POZ) domain. Due to alternative splicing events, KBTBD10 exists as two isoforms.
