MEK-2 Antibody (A-1): sc-13159

MEK-2 Antibody (A-1) is a mouse monoclonal IgG2a kappa light chain antibody, engineered to detect Mitogen-Activated Protein Kinase Kinase 2 (MEK-2), also known as MAP2K2, in mouse, rat, and human samples. MEK-2 monoclonal antibody (A-1) targets an epitope mapping between amino acids 6-25 at the N-terminus of human MEK-2, ensuring precise and reliable binding across various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin embedded sections (IHC-P), and enzyme-linked immunosorbent assay (ELISA). MEK-2 plays a critical role in the MAPK/ERK signaling pathway, functioning as a dual-specificity kinase that phosphorylates and activates ERK1/2, which are essential for regulating cell division, differentiation, and survival. The proper functioning of MEK-2 is vital for normal cellular processes and its dysregulation is implicated in various cancers and other diseases, making accurate detection important for both research and clinical diagnostics. MEK-2 monoclonal antibody (A-1) is available in both conjugated and unconjugated forms, including conjugates with agarose, HRP, PE, FITC, and multiple Alexa Fluor® dyes, providing versatility for different experimental setups and detection systems. MEK-2 monoclonal antibody (A-1) is recommended for researchers studying signal transduction, cancer biology, and therapeutic development.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MEK-2 Antibody (A-1) References:

1. The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product.  |  Crews, CM., et al. 1992. Science. 258: 478-80. PMID: 1411546
2. MEK-2, a Caenorhabditis elegans MAP kinase kinase, functions in Ras-mediated vulval induction and other developmental events.  |  Wu, Y., et al. 1995. Genes Dev. 9: 742-55. PMID: 7729690
3. Components of a new human protein kinase signal transduction pathway.  |  Zhou, G., et al. 1995. J Biol Chem. 270: 12665-9. PMID: 7759517
4. Independent human MAP-kinase signal transduction pathways defined by MEK and MKK isoforms.  |  Dérijard, B., et al. 1995. Science. 267: 682-5. PMID: 7839144
5. Identification and characterization of a new mammalian mitogen-activated protein kinase kinase, MKK2.  |  Wu, J., et al. 1993. Mol Cell Biol. 13: 4539-48. PMID: 8393135
6. Characterization of the structure and function of a novel MAP kinase kinase (MKK6).  |  Han, J., et al. 1996. J Biol Chem. 271: 2886-91. PMID: 8621675
7. Characterization of the structure and function of a new mitogen-activated protein kinase (p38beta).  |  Jiang, Y., et al. 1996. J Biol Chem. 271: 17920-6. PMID: 8663524
8. Chemotactic peptide-induced activation of MEK-2, the predominant isoform in human neutrophils. Inhibition by wortmannin.  |  Downey, GP., et al. 1996. J Biol Chem. 271: 21005-1011. PMID: 8702863
9. Mutation analysis of the coding sequences of MEK-1 and MEK-2 genes in human lung cancer cell lines.  |  Bansal, A., et al. 1997. Oncogene. 14: 1231-4. PMID: 9121773
10. Mitogen-activated protein kinase kinase 7 is an activator of the c-Jun NH2-terminal kinase.  |  Tournier, C., et al. 1997. Proc Natl Acad Sci U S A. 94: 7337-42. PMID: 9207092
11. MKK7 is a stress-activated mitogen-activated protein kinase kinase functionally related to hemipterous.  |  Holland, PM., et al. 1997. J Biol Chem. 272: 24994-8. PMID: 9312105
12. Molecular cloning and characterization of human JNKK2, a novel Jun NH2-terminal kinase-specific kinase.  |  Wu, Z., et al. 1997. Mol Cell Biol. 17: 7407-16. PMID: 9372971

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MEK-2 Antibody (A-1) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-MEK-2 Antibody (A-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MEK-2 Antibody (A-1) Specifications

Alternate Product Name: MEK2

Product Clone Name: A-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 6-25

Epitope Target: MEK-2

Epitope Species: human

Product Reactivity/Detection: MEK-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for MEK-2 Antibody (A-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred MEK-1/2 antibody is MEK-1/2 Antibody (9G3): sc-81504.