Cdc27 Antibody (AF3.1): sc-9972
- Cdc27 Antibody (AF3.1) is a mouse monoclonal IgG2b κ Cdc27 antibody, cited in 73 publications, provided at 200 µg/ml
- raised against amino acids 814-823 of Cdc27 of human origin
- Cdc27 Antibody (AF3.1) is recommended for detection of Cdc27 of mouse, rat, human and Xenopus origin by WB, IP, IF and IHC(P); also reactive with additional species, including and equine and canine
- Anti-Cdc27 Antibody (AF3.1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cdc27 Antibody (AF3.1) for WB and IHC(P) applications. These reagents are now offered in bundles with Cdc27 Antibody (AF3.1) (see ordering information below).
Cdc27 Antibody (AF3.1) is a mouse monoclonal IgG2b kappa light chain antibody that detects Cdc27 protein of mouse, rat, human, and Xenopus laevis origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry (IHC). Cdc27 monoclonal antibody (AF3.1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. Cdc27 plays a crucial role in the regulation of the cell cycle, particularly as a component of the anaphase-promoting complex (APC), which is essential for the timely degradation of cyclins and other regulatory proteins. This degradation is vital for the transition between different phases of the cell cycle, ensuring that cells divide correctly and at the appropriate time. By facilitating the ubiquitination of cyclin B, Cdc27 helps to trigger the metaphase-anaphase transition, thereby preventing errors in chromosome segregation that could lead to aneuploidy or other cell cycle-related disorders. Proper functioning of Cdc27 and the APC is therefore critical for maintaining genomic stability and preventing tumorigenesis, making Cdc27 (AF3.1) antibody an important tool for researchers studying cell cycle regulation and its implications in cancer biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Cdc27 Antibody (AF3.1) References:
- Depletion of anaphase-promoting complex or cyclosome (APC/C) subunit homolog APC1 or CDC27 of Trypanosoma brucei arrests the procyclic form in metaphase but the bloodstream form in anaphase. | Kumar, P. and Wang, CC. 2005. J Biol Chem. 280: 31783-91. PMID: 15994309
- RNA interference knockdown of hU2AF35 impairs cell cycle progression and modulates alternative splicing of Cdc25 transcripts. | Pacheco, TR., et al. 2006. Mol Biol Cell. 17: 4187-99. PMID: 16855028
- cdc25 is a specific tyrosine phosphatase that directly activates p34cdc2. | Gautier, J., et al. 1991. Cell. 67: 197-211. PMID: 1913817
- Phosphorylation of the anaphase-promoting complex/Cdc27 is involved in TGF-beta signaling. | Zhang, L., et al. 2011. J Biol Chem. 286: 10041-50. PMID: 21209074
- Mitotic transmission of artificial chromosomes in cdc mutants of the yeast, Saccharomyces cerevisiae. | Palmer, RE., et al. 1990. Genetics. 125: 763-74. PMID: 2204582
- Identification of a novel protein interaction between Elmo1 and Cdc27. | Lee, J., et al. 2016. Biochem Biophys Res Commun. 471: 497-502. PMID: 26882976
- CDC27 Induces Metastasis and Invasion in Colorectal Cancer via the Promotion of Epithelial-To-Mesenchymal Transition. | Qiu, L., et al. 2017. J Cancer. 8: 2626-2635. PMID: 28900500
- A new twist to the cell cycle. | Barinaga, M. 1995. Science. 269: 631-2. PMID: 7624789
- A 20S complex containing CDC27 and CDC16 catalyzes the mitosis-specific conjugation of ubiquitin to cyclin B. | King, RW., et al. 1995. Cell. 81: 279-88. PMID: 7736580
- Cloning of the human homolog of the CDC34 cell cycle gene by complementation in yeast. | Plon, SE., et al. 1993. Proc Natl Acad Sci U S A. 90: 10484-8. PMID: 8248134
- Mammalian p50Cdc37 is a protein kinase-targeting subunit of Hsp90 that binds and stabilizes Cdk4. | Stepanova, L., et al. 1996. Genes Dev. 10: 1491-502. PMID: 8666233
- A human protein related to yeast Cdc6p. | Williams, RS., et al. 1997. Proc Natl Acad Sci U S A. 94: 142-7. PMID: 8990175
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cdc27 Antibody (AF3.1) | sc-9972 | 200 µg/ml | $322.00 | |||
Cdc27 Antibody (AF3.1): m-IgGκ BP-HRP Bundle | sc-520568 | 200 µg Ab, 40 µg BP | $361.00 | |||
Cdc27 Antibody (AF3.1): m-IgG2b BP-HRP Bundle | sc-548836 | 200 µg Ab; 10 µg BP | $361.00 | |||
Cdc27 Antibody (AF3.1) AC | sc-9972 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Cdc27 Antibody (AF3.1) HRP | sc-9972 HRP | 200 µg/ml | $322.00 | |||
Cdc27 Antibody (AF3.1) FITC | sc-9972 FITC | 200 µg/ml | $336.00 | |||
Cdc27 Antibody (AF3.1) PE | sc-9972 PE | 200 µg/ml | $349.00 | |||
Cdc27 Antibody (AF3.1) Alexa Fluor® 488 | sc-9972 AF488 | 200 µg/ml | $364.00 | |||
Cdc27 Antibody (AF3.1) Alexa Fluor® 546 | sc-9972 AF546 | 200 µg/ml | $364.00 | |||
Cdc27 Antibody (AF3.1) Alexa Fluor® 594 | sc-9972 AF594 | 200 µg/ml | $364.00 | |||
Cdc27 Antibody (AF3.1) Alexa Fluor® 647 | sc-9972 AF647 | 200 µg/ml | $364.00 | |||
Cdc27 Antibody (AF3.1) Alexa Fluor® 680 | sc-9972 AF680 | 200 µg/ml | $364.00 | |||
Cdc27 Antibody (AF3.1) Alexa Fluor® 790 | sc-9972 AF790 | 200 µg/ml | $364.00 |