¡Bienvenido a Santa Cruz Biotechnology!
SCBT Home
Página Principal » Sintesis y Degradación » Ubiquitin Anticuerpos » Ub Antibody (P4D1): sc-8017

Ub Anticuerpo(P4D1): sc-8017

 |  Ficha Técnica

(Basado en el análisis de datos)

  • Ub Antibody (P4D1) is a mouse monoclonal IgG1 provided at 200 µg/ml
  • raised against amino acids 1-76 representing full length Ub of bovine origin
  • recommended for detection of ubiquitin, polyubiquitin and ubiquitinated proteins in multiple species by WB, IP, IF, IHC(P), FCM and ELISA
  • agarose conjugate for IP studies, sc-8017 AC, 500 µg/0.25 ml agarose
  • HRP conjugate, sc-8017 HRP, 200 µg/ml
  • fluorescein (sc-8017 FITC) and phycoerythrin (sc-8017 PE) conjugates for intracellular FCM, 100 tests
  • Alexa Fluor® 405 (sc-8017 AF405), Alexa Fluor® 488 (sc-8017 AF488) and Alexa Fluor® 647 (sc-8017 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
  • See 321 citations for Ub Anticuerpo (P4D1)
 

Ver tambiénUbiquitin AntibodiesincluyebdoUb+1, Ubiquitin, UbyUSPL1.

También verUb Inhibitors for functional analysis of cellular responses to Ub.


Información sobre pedidosMenciones del Producto
Productos Complementarios Recomendados:
(Haga click en el botón de la aplicación que desee)
WB   IP   IF   IHC(P)   FCM   siRNA  
Indique que divisas

 Información sobre pedidos
Nombre del productoNúmero de catálogoUnidadPrecioCantidadAñadir 
Ub Antibody (P4D1) sc-8017 200 µg/ml $279
Ub Antibody (P4D1) AC sc-8017 AC 500 µg/ml, 25% ag $366
Ub Antibody (P4D1) Alexa Fluor® 405 sc-8017 AF405 100 µg/2 ml $314
Ub Antibody (P4D1) Alexa Fluor® 488 sc-8017 AF488 100 µg/2 ml $314
Ub Antibody (P4D1) Alexa Fluor® 647 sc-8017 AF647 100 µg/2 ml $314
Ub Antibody (P4D1) FITC sc-8017 FITC 100 tests in 2 ml $292
Ub Antibody (P4D1) HRP sc-8017 HRP 200 µg/ml $279
Ub Antibody (P4D1) PE sc-8017 PE 100 tests in 2 ml $303
 Silenciadores de Genes siRNA (Haga click en el nombre del producto para obtener más información)
Nombre del productoNúmero de catálogoUnidadPrecioCantidadAñadir 
Ub siRNA (h) sc-29513 10 µM $258
Ub siRNA (m) sc-36770 10 µM $258
Ub (h)-PR sc-29513-PR 10 µM, 20 µl $23
Ub (m)-PR sc-36770-PR 10 µM, 20 µl $23
 Plásmidos shRNA (Haga click en el nombre del producto para obtener más información)
Nombre del productoNúmero de catálogoUnidadPrecioCantidadAñadir 
Ub shRNA Plasmid (h) sc-29513-SH 20 µg $520
Ub shRNA Plasmid (m) sc-36770-SH 20 µg $520
 shRNA partículas antivirales (Haga click en el nombre del producto para obtener más información)
Nombre del productoNúmero de catálogoUnidadPrecioCantidadAñadir 
Ub shRNA (h) Lentiviral Particles sc-29513-V 200 µl $625
Ub shRNA (m) Lentiviral Particles sc-36770-V 200 µl $625
 Lisado de células para control positivo de WB (Haga click en el nombre del producto para obtener más información)
Nombre del productoNúmero de catálogoUnidadPrecioCantidadAñadir 
Ub (h): 293T Lysate sc-111402 100 µg/200 µl $205
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
Jurkat Whole Cell Lysate sc-2204 500 µg/200 µl $104
NIH/3T3 Whole Cell Lysate sc-2210 500 µg/200 µl $104
Jurkat + LLnL Cell Lysate sc-24715 500 µg/200 µl $104
MCF7 Whole Cell Lysate sc-2206 500 µg/200 µl $104
rat brain extract sc-2392 500 µg/200 µl $104

Ubiquitin (Ub) is among the most phylogenetically conserved proteins known. The primary function of ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome. This small, 76 amino acid protein can be covalently attached to cellular proteins via an isopeptide linkage between the carboxy terminal group of ubiquitin and lysine amino groups on the acceptor protein. For proteolysis to occur, ubiquitin oligomers must be assembled. Ubiquitin chains on proteolytic substrates are commonly found to have an isopeptide bridge between Lysine 48 of one ubiquitin molecule and the carboxy-terminus of a neighboring ubiquitin molecule. Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IkBa and p27.

Ub Antibody (P4D1) Menciones del Producto
Vea como otros han usado anticuerpos %catalog_number% y/o anticuerpos conjugados Ub (P4D1).


321 menciones totales
Cargando las citaciones
 
Ub Antibody (P4D1) Data
Haga click en la imágen para agrandarla
sc-8017
Ub (P4D1): sc-8017. Western blot analysis of lysates from control Jurkat cells (A) and Jurkat cells treated with the proteasome inhibitor Lactacystin (sc-3575), showing accumulation of ubiquitinated proteins (B).
sc-8017
Ub (P4D1) PE: sc-8017 PE. Intracellular FCM analysis of fixed and permeabilized NIH/3T3 cells. Black line histogram represents the isotype control, normal mouse IgG1: sc-2855.
sc-8017
Ub (P4D1) FITC : sc-8017 FITC. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG1: sc-2855.
sc-8017
Ub (P4D1): sc-8017. Immunofluorescense staining of methanol-fixed HeLa cells showing weak nuclear staining (A) and strong nuclear staining after treatment with the proteasome inhibitor Lactacystin (sc-3575), showing accumulation of ubiquitinated proteins (B).
sc-8017
Ub (P4D1) Alexa Fluor 488: sc-8017 AF488. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG1: sc-3890.
sc-8017
Ub (P4D1): sc-8017. Western blot analysis of Ub expression in non-transfected: sc-117752 (A) and human Ub transfected: sc-111402 (B) 293T whole cell lysates.
sc-8017
Ub (P4D1) AF647: sc-8017 AF647. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG1: sc-24636.
sc-8017
Ub (P4D1) FITC: sc-8017 FITC. Intracellular FCM analysis of fixed and permeabilized NIH/3T3 cells. Black line histogram represents the isotype control, normal mouse IgG1: sc-2855.
sc-8017
Ub (P4D1): sc-8017. Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing nuclear staining of glandular cells.
sc-8017
Ub (P4D1): sc-8017. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear localization of ubiquitin.
sc-8017
Ub (P4D1): sc-8017. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing nuclear accumulation of ubiquitinated proteins.
Descargar
Ficha técnica
Conseguir Adobe Acrobat Reader