Bcl-2 Antibody (C-2): sc-7382

Bcl-2 Antibody (C-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Bcl-2 protein of mouse, rat, and human origin through western blotting, immunoprecipitation, immunofluorescence, immunohistochemistry, and flow cytometry. Bcl-2 monoclonal antibody (C-2) is offered in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. Bcl-2 protein plays a crucial role as a key regulator of apoptosis, which is vital for normal development, maintaining tissue homeostasis, and providing defense against pathogens. Immunostaining of human tissues reveals that Bcl-2 is primarily localized in the cytoplasm and associated with the membrane, highlighting its function as an anti-apoptotic oncoprotein. Bcl-2 promotes cell survival by engaging in protein-protein interactions with other members of the Bcl-2 family, including the anti-apoptotic proteins Bcl-xL, Mcl-1, Bcl-w, and A1, as well as the pro-apoptotic proteins Bax, Bak, Bik, Bad, and Bid. Regulation of Bcl-2′s anti-apoptotic function can occur through mechanisms such as proteolytic processing and phosphorylation, which are essential for its activity. Additionally, Bcl-2 may inhibit the activation of the cytochrome c/Apaf-1 pathway by stabilizing the mitochondrial membrane, thereby preventing apoptosis. Mutations in the Bcl-2 gene have been implicated in various cancers, where normal apoptotic processes are disrupted due to the overexpression or deregulation of Bcl-2′s anti-apoptotic effects.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Bcl-2 Antibody (C-2) References:

1. The apoptotic v-cyclin-CDK6 complex phosphorylates and inactivates Bcl-2.  |  Ojala, PM., et al. 2000. Nat Cell Biol. 2: 819-25. PMID: 11056537
2. Bcl-2 family proteins as targets for anticancer drug design.  |  Huang, Z. 2000. Oncogene. 19: 6627-31. PMID: 11426648
3. Overexpressed full-length human BCL2 extends the survival of baculovirus-infected Sf9 insect cells.  |  Alnemri, ES., et al. 1992. Proc Natl Acad Sci U S A. 89: 7295-9. PMID: 1502141
4. Role of Bcl-2 family members in anoxia induced cell death.  |  Shroff, EH., et al. 2007. Cell Cycle. 6: 807-9. PMID: 17377500
5. Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death.  |  Hockenbery, D., et al. 1990. Nature. 348: 334-6. PMID: 2250705
6. Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics.  |  Kerr, JF., et al. 1972. Br J Cancer. 26: 239-57. PMID: 4561027
7. Bcl-2 and the regulation of programmed cell death.  |  Reed, JC. 1994. J Cell Biol. 124: 1-6. PMID: 8294493
8. Prevention of apoptosis by Bcl-2: release of cytochrome c from mitochondria blocked.  |  Yang, J., et al. 1997. Science. 275: 1129-32. PMID: 9027314
9. The Bcl-2 protein family: arbiters of cell survival.  |  Adams, JM. and Cory, S. 1998. Science. 281: 1322-6. PMID: 9735050

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Bcl-2 Antibody (C-2) has 2,352 citations in a variety of scientific publications.

Primary antibodies like Anti-Bcl-2 Antibody (C-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Bcl-2 Antibody (C-2) Specifications

Alternate Product Name: Bcl-2

Product Clone Name: C-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-205

Epitope Target: Bcl-2

Epitope Species: human

Product Reactivity/Detection: Bcl-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2,352 citations in various scientific publications

Available Conjugates/Forms for Bcl-2 Antibody (C-2): AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, TRITC: Rhodamine