PRDM1/Blimp-1 Antibody (6D3): sc-47732

Blimp-1 Antibody (6D3) is a rat monoclonal IgG2a antibody that detects Blimp-1 protein of mouse, rat, and, to a lesser extent, human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Blimp-1 (6D3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Blimp-1, also known as B lymphocyte-induced maturation protein or PRDM1, plays a crucial role in the development and differentiation of plasma cells, which are the terminally differentiated form of B-cells responsible for antibody production. This transcriptional repressor is primarily localized in the nucleus, where Blimp-1 acts as a master regulator of terminal B-cell differentiation. Blimp-1 is essential for the upregulation of syndecan-1 and J chain, while simultaneously repressing the transcription of the IFN-β gene and recruiting histone deacetylase to DNA, thereby inhibiting c-myc expression. Blimp-1 expression marks a critical checkpoint in B-cell maturation, allowing fully activated B cells to transition into plasma cells, while eliminating immature and partially activated cells. Understanding Blimp-1 function is vital, as Blimp-1 not only influences the immune response but also has implications in various diseases, including autoimmune disorders and cancers, where dysregulation of plasma cell differentiation can occur.

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PRDM1/Blimp-1 Antibody (6D3) References:

1. Reversal of Blimp-1-mediated apoptosis by A1, a member of the Bcl-2 family.  |  Knödel, M., et al. 1999. Eur J Immunol. 29: 2988-98. PMID: 10508273
2. Transcriptional repression by blimp-1 (PRDI-BF1) involves recruitment of histone deacetylase.  |  Yu, J., et al. 2000. Mol Cell Biol. 20: 2592-603. PMID: 10713181
3. BCL-6 represses genes that function in lymphocyte differentiation, inflammation, and cell cycle control.  |  Shaffer, AL., et al. 2000. Immunity. 13: 199-212. PMID: 10981963
4. Commitment of B lymphocytes to a plasma cell fate is associated with Blimp-1 expression in vivo.  |  Angelin-Duclos, C., et al. 2000. J Immunol. 165: 5462-71. PMID: 11067898
5. Blimp-1; immunoglobulin secretion and the switch to plasma cells.  |  Sciammas, R. and Davis, MM. 2005. Curr Top Microbiol Immunol. 290: 201-24. PMID: 16480044
6. Blimp-1 is a tumor suppressor gene in lymphoid malignancies.  |  Hangaishi, A. and Kurokawa, M. 2010. Int J Hematol. 91: 46-53. PMID: 20047096
7. Immunological function of Blimp-1 in dendritic cells and relevance to autoimmune diseases.  |  Kim, SJ. 2015. Immunol Res. 63: 113-20. PMID: 26376898
8. Blimp-1 is essential for allergen-induced asthma and Th2 cell development in the lung.  |  He, K., et al. 2020. J Exp Med. 217: PMID: 32399548
9. Mononuclear phagocyte regulation by the transcription factor Blimp-1 in health and disease.  |  Ulmert, I., et al. 2020. Immunology. 161: 303-313. PMID: 32799350
10. Blimp-1 molds the epigenetic architecture of IL-21-mediated autoimmune diseases through an autoregulatory circuit.  |  Liu, YW., et al. 2022. JCI Insight. 7: PMID: 35503415
11. Blimp-1, a novel zinc finger-containing protein that can drive the maturation of B lymphocytes into immunoglobulin-secreting cells.  |  Turner, CA., et al. 1994. Cell. 77: 297-306. PMID: 8168136
12. Differential effect of B lymphocyte-induced maturation protein (Blimp-1) expression on cell fate during B cell development.  |  Messika, EJ., et al. 1998. J Exp Med. 188: 515-25. PMID: 9687529

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PRDM1/Blimp-1 Antibody (6D3) has 67 citations in a variety of scientific publications.

Primary antibodies like Anti-PRDM1/Blimp-1 Antibody (6D3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PRDM1/Blimp-1 Antibody (6D3) Specifications

Alternate Product Name: PRDM1

Product Clone Name: 6D3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a

Amino Acids: 255-395

Epitope Target: Blimp-1

Epitope Species: mouse

Product Reactivity/Detection: Blimp-1

Species Reactivity/Detection: mouse, rat

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2a in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 67 citations in various scientific publications

Available Conjugates/Forms for PRDM1/Blimp-1 Antibody (6D3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent