JIP-3 Antibody (F-6): sc-46663

JIP-3 Antibody (F-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects JIP-3 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). JIP-3 (F-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. JIP-3, also known as JSAP1 or MAPK8IP3, is a 1,336 amino acid scaffold protein that plays a crucial role in regulating the c-Jun N-terminal kinase (JNK) signaling pathway, which is vital for cellular responses to stress and apoptosis. Located primarily in the cytoplasm, JIP-3 interacts with various components of the JNK signaling cascade, facilitating the assembly of signaling complexes essential for effective signal transduction. This interaction is particularly important in neuronal cells, where JIP-3 is involved in kinesin-dependent axonal transport, thereby influencing neuronal development and function. JIP-3 can undergo phosphorylation in response to DNA damage, indicating a potential role in cellular stress responses. Human JIP-3 shares 69% sequence similarity with mouse JIP-3, highlighting evolutionary conservation across species. Multiple isoforms of JIP-3 exist due to alternative splicing events, suggesting a complex regulatory mechanism that may fine-tune activity in different cellular contexts.

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JIP-3 Antibody (F-6) References:

1. JSAP1, a novel jun N-terminal protein kinase (JNK)-binding protein that functions as a Scaffold factor in the JNK signaling pathway.  |  Ito, M., et al. 1999. Mol Cell Biol. 19: 7539-48. PMID: 10523642
2. Interaction of a mitogen-activated protein kinase signaling module with the neuronal protein JIP3.  |  Kelkar, N., et al. 2000. Mol Cell Biol. 20: 1030-43. PMID: 10629060
3. Kinesin-dependent axonal transport is mediated by the sunday driver (SYD) protein.  |  Bowman, AB., et al. 2000. Cell. 103: 583-94. PMID: 11106729
4. Expression of JNK cascade scaffold protein JSAP1 in the mouse nervous system.  |  Akechi, M., et al. 2001. Neurosci Res. 39: 391-400. PMID: 11274738
5. Phosphorylation-dependent scaffolding role of JSAP1/JIP3 in the ASK1-JNK signaling pathway. A new mode of regulation of the MAP kinase cascade.  |  Matsuura, H., et al. 2002. J Biol Chem. 277: 40703-9. PMID: 12189133
6. N-terminal kinase, and c-Src are activated in human aortic smooth muscle cells by pressure stress.  |  Morita, N., et al. 2004. Mol Cell Biochem. 262: 71-8. PMID: 15532711
7. Cross-talk between JIP3 and JIP1 during glucose deprivation: SEK1-JNK2 and Akt1 act as mediators.  |  Song, JJ. and Lee, YJ. 2005. J Biol Chem. 280: 26845-55. PMID: 15911620
8. JSAP1/JIP3 cooperates with focal adhesion kinase to regulate c-Jun N-terminal kinase and cell migration.  |  Takino, T., et al. 2005. J Biol Chem. 280: 37772-81. PMID: 16141199
9. Identification of ROCK1 as an upstream activator of the JIP-3 to JNK signaling axis in response to UVB damage.  |  Ongusaha, PP., et al. 2008. Sci Signal. 1: ra14. PMID: 19036714
10. Syd/JIP3 controls tissue size by regulating Diap1 protein turnover downstream of Yorkie/YAP.  |  Ahmad, V., et al. 2021. Dev Biol. 469: 37-45. PMID: 33022230
11. JIP3 links lysosome transport to regulation of multiple components of the axonal cytoskeleton.  |  Rafiq, NM., et al. 2022. Commun Biol. 5: 5. PMID: 35013510
12. JIP3 interacts with dynein and kinesin-1 to regulate bidirectional organelle transport.  |  Celestino, R., et al. 2022. J Cell Biol. 221: PMID: 35829703

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-JIP-3 Antibody (F-6) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-JIP-3 Antibody (F-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

JIP-3 Antibody (F-6) Specifications

Alternate Product Name: JIP-3

Product Clone Name: F-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 211-350

Epitope Target: JIP-3

Epitope Species: human

Product Reactivity/Detection: JIP-3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for JIP-3 Antibody (F-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin