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AmpliCruz™: sc-45049

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  • Materials for 15-20 enhancements when used as directed
  • The AmpliCruzTM Western Blot Signal Enhancement System is designed to amplify the desired signal detected by Western blot, and is an excellent tool for evaluating proteins with low expression.
  • Membrane Enhancement Solution is used to pre-treat membrane prior to blocking the membrane; 1x solution; 250 ml
  • Membrane Blocking Solution is used to block the pre-treated membrane; 1x solution; 250 ml
  • Primary/Secondary Dilution Buffer is used to dilute primary WB antibody and the secondary detection reagent; 5x solution; 100 ml
  • Store at room temperature
  • See 1 citations for AmpliCruz™
 
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AmpliCruzTM sc-45049 1 kit $76
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AmpliCruz™ Data
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sc-45049
Western blot analysis of Renin expression in A-10 whole cell lysate using standard Western blotting protocols (A,B) and AmpliCruz Western Blot Enhancement System: sc-45049 (C). Primary antibody used at a 1:100 dilution. Secondary antibody used at 1:4000 (A) and 1:32000 (B,C) dilutions. Note presence of stronger signal using less secondary antibody when AmpliCruz is used.
sc-45049
Western blot analysis of MEK-1 expression in HeLa whole cell lysate using standard Western blotting protocols (A) and AmpliCruz Western Blot Enhancement System: sc-45049 (B). Primary antibody used at a 1:100 dilution. Secondary antibody used at 1:32000 dilution. Note presence of stronger signal when AmpliCruz is used.
sc-45049
Western blot analysis of SCCA1/2 expression in SCC-4 whole cell lysate using standard Western blotting protocols (A,B) and AmpliCruz Western Blot Enhancement System: sc-45049 (C). Primary antibody used at a 1:100 dilution. Secondary antibody used at 1:2500 (A) and 1:32000 (B,C) dilutions. Note presence of stronger signal using less secondary antibody when AmpliCruz is used.
sc-45049
Western blot analysis of Cdk2 expression in A-431 whole cell lysate using standard Western blotting protocols (A) and AmpliCruz Western Blot Enhancement System: sc-45049 (B). Primary antibody used at a 1:100 dilution. Secondary antibody used at 1:32000 dilution. Note presence of stronger signal when AmpliCruz is used.
sc-45049
Western blot analysis of p53 expression in LacZ whole cell lysate using standard Western blotting protocols (A-D) and AmpliCruz Western Blot Enhancement System: sc-45049 (E,F). Primary antibody used at 1:100 (A,B) and 1:1000 (C-F) dilutions. Secondary antibody used at 1:4000 (A,B) and 1:32000 (C-F) dilutions. Note presence of signal and less background using less primary and secondary antibodies when AmpliCruz is used.
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