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Caspase-8 Kit: sc-4267 AK

 | Datasheets: sc-4267 AK

(Basado en popularidad)

  • sufficient for 50 tests
  • target sequence: IETD
  • recommended for the fluorometric detection of specific caspase activities
  • utilizes tetrapeptide substrates, which are recognized by specific caspases, conjugated to the fluorophore AFC (7-amino-4-trifluoromethyl coumarin)
  • IETD-AFC acts as a synthetic substrate for caspase-8
  • Cleavage of the substrate may be monitored on a fluorometer or a fluorescence microplate reader; comparison of the readings with apoptotic vs. control lysates permits determination of relative caspase activity.
  • Kit includes substrate, cell lysis buffer, 2x reaction buffer and DTT.
 
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Caspase-8 Kit sc-4267 AK 500 µl $205