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goat anti-mouse IgG, F(ab’)2-APC: sc-3818

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  • supplied at 200 µg in 0.5 ml volume
  • F(ab’)2 fragment secondary antibody
  • allophycocyanin conjugated
  • to be used in immunohistochemical staining at a dilution of 1:100 - 1:400
  • F(ab’)2 fragment secondary antibodies should be used when trying to avoid non-specific secondary antibody binding to Fc receptors on cell surfaces
  • pre-adsorbed with human IgG
  • See 1 citations for goat anti-mouse IgG, F(ab’)2-APC
 
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goat anti-mouse IgG, F(ab’)2-APC sc-3818 200 µg/0.5 ml $100

Santa Cruz Biotechnology’s secondary antibodies are available conjugated to either an enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, immunostaining, flow cytometry and ELISA. Secondary antibodies are commonly affinity purified against immobilized whole IgG or against antibody fragments such as the Fc or F(ab')2 regions. Santa Cruz Biotechnology offers an extensive selection of F(ab')2 specific secondary antibodies for immunohistochemistry and flow cytometry that are non-conjugated or labeled with either AP (alkaline phosphatase), fluorescein, biotin, FITC (fluorescein isothiocyanate), Texas Red®, TRITC (tetramethyl rhodamine iso-thiocyanate), PE (phycoerythrin), PE-Cy5 (phycoerythrin with cyanin-5), PE-Cy7 (phycoerythrin with cyanin-7), APC (allophycocyanin), APC-Cy7 and (allophycocyanin with cyanin-7). F(ab')2 secondary antibodies are specific for commonly used primary antibody species, including goat, rabbit, mouse and rat, and are recommended for reducing non-specific secondary antibody binding to Fc receptors on the cell surface.

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goat anti-mouse IgG, F(ab’)2-APC Data
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sc-3818
cytochrome c (7H8): sc-13560. Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with cytochrome c (7H8), followed by APC-conjugated goat anti-mouse IgG F(ab')2: sc-3818. Black line histogram represents the isotype control, normal mouse IgG: sc-2025.
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