Lamin B1 Antibody (A-11): sc-377000
- Lamin B1 Antibody (A-11) is a mouse monoclonal IgG1 κ Lamin B1 antibody, cited in 110 publications, provided at 200 µg/ml
- raised against amino acids 401-490 mapping near the C-terminus of Lamin B1 of human origin
- Lamin B1 Antibody (A-11) is recommended for detection of Lamin B1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-Lamin B1 Antibody (A-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Lamin B1 Antibody (A-11) for WB and IHC(P) applications. These reagents are now offered in bundles with Lamin B1 Antibody (A-11) (see ordering information below).
Lamin B1 Antibody (A-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects Lamin B1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Lamin B1 antibody (A-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Lamin B1, a 586 amino acid protein encoded by the LMNB1 gene, plays a crucial role in maintaining nuclear envelope structural integrity and cellular morphology as a key component of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane. This structural framework provides stability to the nuclear envelope and may facilitate interactions with chromatin, influencing gene expression and cellular function. Mutations in the LMNB1 gene have been linked to autosomal dominant adult-onset leukodystrophy (ADLD), a neurodegenerative disorder characterized by cerebellar dysfunction and demyelination of the central nervous system, highlighting Lamin B1′s significance in cellular health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Lamin B1 Antibody (A-11) References:
- The dynamic properties and possible functions of nuclear lamins. | Moir, RD., et al. 1995. Int Rev Cytol. 162B: 141-82. PMID: 8557486
- ICE-LAP3, a novel mammalian homologue of the Caenorhabditis elegans cell death protein Ced-3 is activated during Fas- and tumor necrosis factor-induced apoptosis. | Duan, H., et al. 1996. J Biol Chem. 271: 1621-5. PMID: 8576161
- ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B. | Duan, H., et al. 1996. J Biol Chem. 271: 16720-4. PMID: 8663294
- Cleavage of lamin A by Mch2 alpha but not CPP32: multiple interleukin 1 beta-converting enzyme-related proteases with distinct substrate recognition properties are active in apoptosis. | Takahashi, A., et al. 1996. Proc Natl Acad Sci U S A. 93: 8395-400. PMID: 8710882
- In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains. | Fernandes-Alnemri, T., et al. 1996. Proc Natl Acad Sci U S A. 93: 7464-9. PMID: 8755496
- Lamin proteolysis facilitates nuclear events during apoptosis. | Rao, L., et al. 1996. J Cell Biol. 135: 1441-55. PMID: 8978814
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Lamin B1 Antibody (A-11) | sc-377000 | 200 µg/ml | $322.00 | |||
Lamin B1 Antibody (A-11): m-IgGκ BP-HRP Bundle | sc-523146 | 200 µg Ab, 40 µg BP | $361.00 | |||
Lamin B1 Antibody (A-11): m-IgG1 BP-HRP Bundle | sc-533558 | 200 µg Ab; 20 µg BP | $361.00 | |||
Lamin B1 Antibody (A-11) AC | sc-377000 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Lamin B1 Antibody (A-11) HRP | sc-377000 HRP | 200 µg/ml | $322.00 | |||
Lamin B1 Antibody (A-11) FITC | sc-377000 FITC | 200 µg/ml | $336.00 | |||
Lamin B1 Antibody (A-11) PE | sc-377000 PE | 200 µg/ml | $349.00 | |||
Lamin B1 Antibody (A-11) Alexa Fluor® 488 | sc-377000 AF488 | 200 µg/ml | $364.00 | |||
Lamin B1 Antibody (A-11) Alexa Fluor® 546 | sc-377000 AF546 | 200 µg/ml | $364.00 | |||
Lamin B1 Antibody (A-11) Alexa Fluor® 594 | sc-377000 AF594 | 200 µg/ml | $364.00 | |||
Lamin B1 Antibody (A-11) Alexa Fluor® 647 | sc-377000 AF647 | 200 µg/ml | $364.00 | |||
Lamin B1 Antibody (A-11) Alexa Fluor® 680 | sc-377000 AF680 | 200 µg/ml | $364.00 | |||
Lamin B1 Antibody (A-11) Alexa Fluor® 790 | sc-377000 AF790 | 200 µg/ml | $364.00 |