TGFα Antibody (D-6): sc-374433

TGFα Antibody (D-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects TGFα protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). TGFα Antibody (D-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Transforming growth factor-α (TGFα) is a crucial 50 amino acid protein that plays a significant role in cell signaling and growth regulation. TGFα binds to the epidermal growth factor receptor, mediating tyrosine phosphorylation of the receptor and promoting anchorage-independent growth of normal rat fibroblasts in soft agar, particularly with transforming growth factor-β. TGFα is secreted by transformed cells, tumors, embryonic cells, and some normal adult cells, highlighting TGFα′s importance in both normal physiology and pathological conditions. TGFα bioactivity has been detected in cancer patients′ urine, suggesting TGFα′s potential role as an autocrine growth factor that may contribute to malignancy induction or maintenance. Understanding TGFα function is vital, as TGFα influences cellular proliferation and has implications in cancer biology and therapeutic strategies.

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TGFα Antibody (D-6) References:

1. Functional interaction between transforming growth factor alpha and capsaicin-sensitive sensory neurons in the rat stomach.  |  Vongthavaravat, V., et al. 2004. Regul Pept. 119: 163-7. PMID: 15120476
2. Evidence for repatterning of the gastric fundic epithelium associated with Ménétrier's disease and TGFalpha overexpression.  |  Nomura, S., et al. 2005. Gastroenterology. 128: 1292-305. PMID: 15887112
3. Preparation and characterization of monoclonal antibodies specific for human transforming growth factor alpha.  |  Sorvillo, JM., et al. 1990. Oncogene. 5: 377-86. PMID: 1690379
4. Differential expression of epidermal growth factor-related proteins in human colorectal tumors.  |  Ciardiello, F., et al. 1991. Proc Natl Acad Sci U S A. 88: 7792-6. PMID: 1715580
5. Involvement of transforming growth factor alpha in the photoperiodic regulation of reproduction in birds.  |  Takagi, T., et al. 2007. Endocrinology. 148: 2788-92. PMID: 17363456
6. Expression of ErbB receptor proteins and TGF-alpha during diethylnitrosamine-induced hepatocarcinogenesis in the rat liver.  |  Lee, TY., et al. 2007. Korean J Hepatol. 13: 70-80. PMID: 17380077
7. The induction of C/EBPβ contributes to vitamin D inhibition of ADAM17 expression and parathyroid hyperplasia in kidney disease.  |  Arcidiacono, MV., et al. 2015. Nephrol Dial Transplant. 30: 423-33. PMID: 25294851
8. Alpha-transforming growth factor secreted by untransformed bovine anterior pituitary cells in culture. I. Purification from conditioned medium.  |  Samsoondar, J., et al. 1986. J Biol Chem. 261: 14408-13. PMID: 3464597
9. Transforming growth factor-alpha: structure and biological activities.  |  Derynck, R. 1986. J Cell Biochem. 32: 293-304. PMID: 3543030
10. Human transforming growth factors induce tyrosine phosphorylation of EGF receptors.  |  Reynolds, FH., et al. 1981. Nature. 292: 259-62. PMID: 6265789
11. Transforming growth factors produced by retrovirus-transformed rodent fibroblasts and human melanoma cells: amino acid sequence homology with epidermal growth factor.  |  Marquardt, H., et al. 1983. Proc Natl Acad Sci U S A. 80: 4684-8. PMID: 6308641
12. Distinct high-performance liquid chromatography pattern of transforming growth factor activity in urine of cancer patients as compared with that of normal individuals.  |  Kimball, ES., et al. 1984. Cancer Res. 44: 3613-9. PMID: 6331653

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TGFα Antibody (D-6) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-TGFα Antibody (D-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TGFα Antibody (D-6) Specifications

Alternate Product Name: TGFA

Product Clone Name: D-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 63-89

Epitope Target: TGFα

Epitope Species: human

Product Reactivity/Detection: presursor and mature TGFα and BTC

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for TGFα Antibody (D-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin