TBST: powder for 5 L of 10X

TBST: powder for 5 L of 10X (Tris Buffered Saline with Tween 20) is commonly used as a wash solution for western blot membranes and microtiter plate wells in ELISA assays. TBST is an optimal formulation of pH stabilizers, salts and detergents designed to effectively remove excess material from the microtiter plate wells without disrupting the ELISA binding reaction. By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background and increasing the specific signal.

Also available without Tween 20:
TBS: 1 L of 1X (sc-362185)
TBS: 1 L of 10X (sc-24951)
TBS: 100 ml of 10X (sc-362303)
TBS: 500 ml of 10X (sc-362304)
TBS: 1 L of 20X (sc-362186)
TBS: 5 L of 20X (sc-362305)
TBS: powder for 100 ml of 10X (sc-362188)
TBS: powder for 500 ml of 10X (sc-362306)
TBS: powder for 1 L of 10X (sc-362307)
TBS: powder for 5 L of 10X (sc-362308)

Reconstitute powder in ddH₂0 and empty contents of Tween-20 vial (rinse vial thoroughly) into liquid, bringing final volume to 100 ml (10X). Filter solution through a 0.22 μm filter into a sterile flask. Makes 1 L of 1X TBST buffer.
Store the buffer solution at +4°C. Final concentration of Tween-20 (1X solution) = 0.05%. Final (1X) pH 7.4 ±0.2 (may need slight pH adjustment).

SDS

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