PBS: 5 L of 20X

PBS: 5 L of 20X (Phosphate buffered saline) is a concentrated buffer solution ready for dilution to a 1X PBS working solution commonly used in biological research. PBS buffer is ideal for maintaining a constant pH, and since it is isotonic and non-toxic to cells, it can be used in a wide variety of biological applications. PBS is frequently used as a wash and dilution buffer, and is also used to take a reference spectrum when measuring protein adsorption.

• Prior to use, dilute to a 1X solution and filter through a 0.22 µm filter into a sterile flask. Makes 100 L of 1X PBS buffer with a phosphate buffer concentration of 0.01M, 0.027M potassium chloride, 0.137M sodium chloride, and 1.76 mM potassium phosphate. The PBS solution pH will be approximately 7.4.
• If buffer is used in cell culture applications, sterilization of the solution may be necessary. Pre-diluted buffer can be dispensed into aliquots and sterilized by autoclaving (20 min, 121°C, liquid cycle).
• PBS can be stored at room temperature, but non-sterile solutions may warrant refrigeration to prevent bacterial growth over time. Concentrated stock solutions may precipitate when cooled and should be kept at room temperature until precipitate has completely dissolved before use.

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