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SK-BR-3 + PMA nuclear extract: sc-2135

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  • supplied in four vials, each containing 250 µg nuclear extract in 50 µl buffer
  • provided in 20 mM HEPES (pH 7.9), 20% v/v glycerol, 0.1 M KCI, 0.2 mM EDTA, 0.5 mM PMSF and 0.5 mM DTT
  • human nuclear extract; breast adenocarcinoma cells induced with phorbol
  • suitable for use in Gel Shift and Western Blotting assays
  • Extracts should be stored at -70°C and repeated freezing and thawing should be avoided.
  • prepared by the method of Dignam et al., (1983) Nucleic Acids Res. 11: 1475
 
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SK-BR-3 + PMA nuclear extract sc-2135 250 µg/0.05 ml $143