Akt1 Anticuerpo(C-20) | Santa Cruz Biotech

Iniciar sesión | 0 productos en su carro | Pedido Rápido

¡Bienvenido a Santa Cruz Biotechnology!

Investigación
Anticuerpos Primarios: Mamíferos Anticuerpos fosfoespecíficos Anticuerpos para tinción de IHC (Parafina) Citometría de Flujo Anticuerpos Investigación Veterinaria Anticuerpos Primarios: No- Mamíferos Productos siRNA y shRNA (plásmido y lentiviral) Proteína A, G PLUS y L-Agarose Extractos de Tejidos y Lisados Celulares Proteinas Biológicamente Activas y Purificadas Lisados de células transfectadas Anticuerpos Secundarios, Anticuerpos Control y Suero Normal Reactivos especiales y Kits
Químicos
Bioquímicos Tampones Preparados Tampones y Químicos Estándar para Laboratorio Tintes Colorantes y Sondas Fluorescentes Disolventes Sustratos HRP y AP
Material laboratorio
Salud Animal
Salud Animal Promoción Salud Animal Promoción libro Noticias Salud Animal Farmacia Salud Animal Complementos Ultra Cruz
Novedades
Promociones LIF de la Proteína Anticuerpos Investigación Veterinaria Anticuerpos Monoclonales Nueva tinción para IHC (Parafina)
Información
Contacto Información sobre pedidos Cruz Credits™ Investigator Awards Ofertas de trabajo Distribuidores
Apoyo
Protocolos Menciones del Producto Ficha técnica / MSDS Servicio técnico Solicitud de Catálogo Cromosoma DBase

Akt1 Anticuerpo(C-20): sc-1618	\| Ficha Técnica

5 5 (Basado en el análisis de datos) 1

Akt1 Antibody (C-20) is a goat polyclonal IgG provided at 200 µg/ml; also available as rabbit IgG provided at 200 µg/ml, sc-1618-R
epitope mapping at the C-terminus of Akt1 of human origin
recommended for detection of Akt1 and, to a lesser extent, Akt2 and Akt3 of mouse, rat, human and Xenopus origin by WB, IP, IF, FCM and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
blocking peptide, sc-1618 P
agarose conjugate for IP studies, sc-1618 AC, 500 µg/0.25 ml agarose
phycoerythrin (sc-1618 PE) conjugate for intracellular FCM, 100 tests
See 164 citations for Akt1 Anticuerpo (C-20)

Ver tambiénAkt AntibodiesincluyebdoAkt, Akt1, Akt2yAkt3.

También verAkt1 InhibitorsoAkt1 Substrates for functional analysis of cellular responses to Akt1.

Información sobre pedidosMenciones del ProductoInformación del gen

Productos Complementarios Recomendados:

(Haga click en el botón de la aplicación que desee)

WB IP IF FCM

Información sobre pedidos

Nombre del producto	Número de catálogo	Unidad	Precio
Akt1 Antibody (C-20)	sc-1618	200 µg/ml	$279
Akt1 Antibody (C-20) AC	sc-1618 AC	500 µg/ml, 25% ag	$366
Akt1 (C-20) P	sc-1618 P (peptide)	100 µg/0.5 ml	$61
Akt1 Antibody (C-20) PE	sc-1618 PE	100 tests in 2 ml	$303
Akt1 Antibody (C-20)-R	sc-1618-R	200 µg/ml	$279

Lisado de células para control positivo de WB (Haga click en el nombre del producto para obtener más información)

Nombre del producto	Número de catálogo	Unidad	Precio
MCF7 Whole Cell Lysate	sc-2206	500 µg/200 µl	$104
KNRK Whole Cell Lysate	sc-2214	500 µg/200 µl	$104
NIH/3T3 Whole Cell Lysate	sc-2210	500 µg/200 µl	$104
HeLa Whole Cell Lysate	sc-2200	500 µg/200 µl	$104
MIA PaCa-2 Cell Lysate	sc-2285	500 µg/200 µl	$104
ZR-75-1 Cell Lysate	sc-2241	500 µg/200 µl	$104
BT-20 Cell Lysate	sc-2223	500 µg/200 µl	$104

Especies	Nombre del Gen	Gene ID	Ubicación en el cromosoma	Numero de Acceso de Isoforma (ARNm)	Número de Acceso de la Proteína	Número OMIM™:

Humano	AKT1	207	14q32.33	NM_001014431, NM_001014432, NM_005163	P31749	604370
Ratón	Akt1	11651	12 F1		P31750	No Disponible
Ratón	Akt2	11652	7 A3		Q60823	No Disponible
Ratón	Akt3	23797	1 H4		Q9WUA6	No Disponible

The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKB∫ or RacPK-∫) and Akt 3 (also designated PKB© or thyoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-∫ tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin, suggesting that the protein signals downstream of the PI kinases.

Akt1 Antibody (C-20) Menciones del Producto

Vea como otros han usado anticuerpos %catalog_number% y/o anticuerpos conjugados Akt1 (C-20).

164 menciones totales

Cargando las citaciones

Akt1 Antibody (C-20) Data

Haga click en la imágen para agrandarla

Akt1 (C-20): sc-1618. Western blot analysis of Akt1 expression in MCF7 (A), KNRK (B) and NIH/3T3 (C) whole cell lysates.

Western blot analysis of Akt1 phosphorylation in A-431 cells treated with EGF. Blots were probed with Akt1 (C-20): sc-1618 (A), p-Akt1/2/3 (Ser 473): sc-7985-R preincubated with cognate unphosphorylated peptide (B) and p-Akt1/2/3 (Ser 473): sc-7985-R preincubated with cognate phosphorylated peptide (C).

Akt1 (C-20)-R: sc-1618-R. Western blot analysis of Akt1 expression in KNRK (A), NIH/3T3 (B), HeLa (C), ZR-75-1 (D) and BT-20 (E) whole cell lysates.

Akt1 (C-20): sc-1618. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

Akt1 (C-20): sc-1618. Western blot analysis of Akt2 expression in non-transfected 293T: sc-117752 (A), human Akt2 transfected 293T: sc-116831 (B) and A-431 (C) whole cell lysates.

Akt1 (C-20): sc-1618. Western blot analysis of Akt2 expression in non-transfected 293T: sc-117752 (A), mouse Akt2 transfected 293T: sc-126407 (B) and KNRK (C) whole cell lysates.

Akt1 (C-20): sc-1618. Western blot analysis of Akt1 expression in IMR-32 whole cell lysate.

Akt1 (C-20): sc-1618. Western blot analysis of Akt1 expression in non-transfected: sc-117752 (A) and human Akt1 transfected: sc-176810 (B) 293T whole cell lysates.

Akt1 (C-20)-R: sc-1618-R. Western blot analysis of Akt1 expression in non-transfected: sc-117752 (A) and human Akt1 transfected: sc-176810 (B) 293T whole cell lysates.

Akt1 (C-20): sc-1618. Western blot analysis of Akt1 expression in non-transfected: sc-117752 (A) and human Akt1 transfected: sc-158248 (B) 293T whole cell lysates.

Western blot analysis of Akt1 phosphorylation in untreated (A,D), serum starved and insulin treated (B,E) and serum starved, insulin treated and lambda protein phosphatase (sc-200312A) treated (C,F) HEK293 whole cell lysates. Antibodies tested include p-Akt1 (11E6): sc-81433 (A,B,C) and Akt1 (C-20): sc-1618 (D,E,F).

Western blot analysis of Akt1 phosphorylation in untreated (A,D), serum starved and insulin treated (B,E) and serum starved, insulin treated and lambda protein phosphatase (sc-200312A) treated (C,F) HEK293 whole cell lysates. Antibodies tested include p-Akt1/2/3 (Ser 473): sc-33437 (A,B,C) and Akt1 (C-20): sc-1618 (D,E,F).

Western blot analysis of Akt1 phosphorylation in untreated (A,D), mouse LIF (sc-4989) treated (B,E) and LIF and lambda protein phosphatase (sc-200312A) treated (C,F) 3T3-L1 whole cell lysates. Antibodies tested include p-Akt1 (11E6): sc-81433 (A,B,C) and Akt1 (C-20): sc-1618 (D,E,F).

Akt1 (C-20) PE: sc-1618 PE. Intracellular FCM analysis of fixed and permeabilized NIH/3T3 cells. Black line histogram represents the isotype control, normal goat IgG: sc-3992.

Western blot analysis of Akt1 phosphorylation in untreated (A,D), mouse LIF treated (B,E), and mouse LIF and lambda protein phosphatase (sc-200312A) treated (C,F) 3T3-L1 whole cell lysates. Antibodies tested include p-Akt1 (104A282): sc-52940 (A,B,C) and Akt1 (C-20): sc-1618 (D,E,F).

Western blot analysis of Akt1 phosphorylation in non-transfected: sc-117752 (A,D), untreated human Akt1 transfected: sc-158248 (B,E) and lambda protein phosphatase (sc-200312A) treated human Akt1 transfected: sc-158248 (C,F) 293T whole cell lysates. Antibodies tested include p-Akt1/2/3 (A-12): sc-271964 (A,B,C) and Akt1 (C-20): sc-1618 (D,E,F).

Western blot analysis of Akt1 phosphorylation in non-transfected: sc-117752 (A,D), untreated human Akt1 transfected: sc-158248 (B,E) and lambda protein phosphatase (sc-200312A) treated human Akt1 transfected: sc-158248 (C,F) 293T whole cell lysates. Antibodies tested include p-Akt1/2/3 (B-5): sc-271966 (A,B,C) and Akt1 (C-20): sc-1618 (D,E,F).

Western blot analysis of Akt1 phosphorylation in non-transfected: sc-117752 (A,D), untreated human Akt1 transfected: sc-158248 (B,E) and lambda protein phosphatase (sc-200312A) treated human Akt1 transfected: sc-158248 (C,F) 293T whole cell lysates. Antibodies tested include p-Akt1 (Thr 308): sc-135650 (A,B,C) and Akt1 (C-20): sc-1618 (D,E,F).

Western blot analysis of Akt1 phosphorylation in untreated (A,D), mLIF treated (B,E) and mLIF and lambda protein phosphatase treated (C,F) 3T3-L1 whole cell lysates. Antibodies tested includep-Akt1 (5.Ser 473): sc-293125 (A,B,C) and Akt1 (C-20): sc-1618 (D,E,F).

Western blot analysis of Akt1 phosphorylation in untreated (A,D), Calyculin A treated (B,E) and Calyculin A and lambda protein phosphatase (sc-200312A) treated (C,F) Jurkat whole cell lysates. Antibodies tested include p-Akt1 (5.Ser 473): sc-293125 (A,B,C) and Akt1 (C-20): sc-1618 (D,E,F).

Descargar

Ficha técnica

Conseguir Adobe Acrobat Reader

Akt1 Anticuerpo(C-20): sc-1618