Hic-5 Antibody (F-6): sc-137051

Hic-5 Antibody (F-6) is a mouse monoclonal IgG2b antibody that detects hydrogen-peroxide inducible clone 5 (Hic-5) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Hic-5 is a crucial member of the LIM family, characterized by four LIM motifs and seven zinc finger domains, which play a significant role in mediating protein-protein interactions within the cell. Hic-5 (F-6) antibody recognizes a protein primarily localized to the nuclear matrix and focal adhesion complexes, where Hic-5 is essential for regulating cellular adhesion and signaling pathways. Notably, Hic-5 inhibits integrin-mediated cell spreading on fibronectin by competing with paxillin for binding to focal adhesion kinase, thereby disrupting downstream signaling cascades vital for cell migration and proliferation. Hic-5 involvement extends to cellular senescence, particularly in developing fibroblasts, where increased expression can lead to growth arrest. During myogenesis, Hic-5 expression blocks differentiation and induces apoptosis in developing myoblasts, highlighting a regulatory role in muscle development. The gene encoding human Hic-5 is located on chromosome 16, underscoring evolutionary significance and potential implications in various biological processes.

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Hic-5 Antibody (F-6) References:

1. Hydrogen peroxide-inducible clone 5 (Hic-5) as a potential therapeutic target for vascular and other disorders.  |  Kim-Kaneyama, JR., et al. 2012. J Atheroscler Thromb. 19: 601-7. PMID: 22472216
2. Hic-5 is required for myofibroblast differentiation by regulating mechanically dependent MRTF-A nuclear accumulation.  |  Varney, SD., et al. 2016. J Cell Sci. 129: 774-87. PMID: 26759173
3. Hic-5 mediates the initiation of endothelial sprouting by regulating a key surface metalloproteinase.  |  Dave, JM., et al. 2016. J Cell Sci. 129: 743-56. PMID: 26769900
4. Hic-5 remodeling of the stromal matrix promotes breast tumor progression.  |  Goreczny, GJ., et al. 2017. Oncogene. 36: 2693-2703. PMID: 27893716
5. Hic-5 regulates Src-induced invadopodia rosette formation and organization.  |  Gulvady, AC., et al. 2019. Mol Biol Cell. 30: 1298-1313. PMID: 30893012
6. HIC-5 in cancer-associated fibroblasts contributes to esophageal squamous cell carcinoma progression.  |  Du, X., et al. 2019. Cell Death Dis. 10: 873. PMID: 31740661
7. Hic-5 deficiency protects cerulein-induced chronic pancreatitis via down-regulation of the NF-κB (p65)/IL-6 signalling pathway.  |  Chen, H., et al. 2020. J Cell Mol Med. 24: 1488-1503. PMID: 31797546
8. Suppressing of Src-Hic-5-JNK-AKT Signaling Reduced GAPDH Expression for Preventing the Progression of HuCCT1 Cholangiocarcinoma.  |  Wu, WS., et al. 2022. Pharmaceutics. 14: PMID: 36559193
9. Knockdown of mechanosensitive adaptor Hic-5 ameliorates post-traumatic osteoarthritis in rats through repression of MMP-13.  |  Miyauchi, A., et al. 2023. Sci Rep. 13: 7446. PMID: 37156857
10. Hic-5 antisense oligonucleotide inhibits advanced hepatic fibrosis and steatosis in vivo.  |  Noguchi, M., et al. 2024. JHEP Rep. 6: 101195. PMID: 39444410

The preferred Hic-5 antibody is Hic-5 Antibody (C-6): sc-271353.