p-5-LO Anticuerpo(Ser 523): sc-135685

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5-lipoxygenase (5-LO) is expressed primarily in polymorphonuclear leukocytes, macrophages, and mast cells (1). 5-LO performs the first two catalytic reactions in the biosynthesis of leukotrienes, lipid metabolites that induce contractions of airway smooth muscle and increase vascular permeability during anaphylaxis (1,2). The cellular localization of 5-LO varies between cell types (3). In activated blood polymorphonuclear leukocytes 5-LO undergoes calcium dependent translocation from the cytosol to the nuclear envelope (4). In alveolar macrophages, the majority of 5-LO is localized in the nucleus and, upon activation of these cells, intranuclear 5-LO binds to the nuclear membrane (5). This intracellular shuttling of 5-LO is dependent on the association with various signaling molecules, phosphorylation and the presence of a distinct nuclear localization signal, which is encoded at the amino terminus of 5-LO (6,7). Human and rat 5-LO are phosphorylated on Ser 523 by PKA which has an inhibitory effect on 5-LO.