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p-ZAP-70 Anticuerpo(Tyr 319)-R: sc-12946-R

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(Basado en el análisis de datos)

  • p-ZAP-70 (Tyr 319)-R Antibody is a rabbit polyclonal IgG provided at 200 µg/ml
  • recommended for detection of Tyr 319 phosphorylated ZAP-70 and Tyr 352 phosphorylated Syk of mouse, rat and human origin by WB, IP, IF and ELISA
  • blocking peptide, sc-12946 P
 

Ver tambiénZAP-70 Antibodies.

Información sobre pedidos
Productos Complementarios Recomendados:
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WB   IP   IF  
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 Información sobre pedidos
Nombre del productoNúmero de catálogoUnidadPrecioCantidadAñadir 
p-ZAP-70 (Tyr 319) P sc-12946 P
(peptide)
 100 µg/0.5 ml $61
p-ZAP-70 (Tyr 319)-R Antibody sc-12946-R 200 µg/ml $279
Lambda Phosphatase sc-200312 100000 units in 0.25 ml $462
Lambda Phosphatase sc-200312A 20000 units in 0.05 ml $116
Lambda Phosphatase sc-200312 100000 units in 0.25 ml $462
Lambda Phosphatase sc-200312A 20000 units in 0.05 ml $116
 Lisado de células para control positivo de WB (Haga click en el nombre del producto para obtener más información)
Nombre del productoNúmero de catálogoUnidadPrecioCantidadAñadir 
Jurkat + hydrogen peroxide Cell Lysate sc-24714 500 µg/200 µl $104
Jurkat Whole Cell Lysate sc-2204 500 µg/200 µl $104

The activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR), which is a multisubunit complex assembled from at least six different genes (1,2). The TCR subunits include the Ti a and b chains, the CD3 g , d and e chains and a z -containing homodimer or heterodimer (1,2). The protein tyrosine kinase ZAP-70 binds to the phosphorylated immunoreceptor tyrosine-base activation motifs (ITAMs) of the TCR z chain through two src-homology (SH2) domains (3,4). This binding results in the phosphorylation of ZAP-70 on multiple tyrosine residues, including Tyr292 and Tyr319 (4). ZAP-70 is autophosphorylated on Tyr292, which is thought to negatively regulate ZAP-70 function in lymphocytes (5,6). Alternatively, ZAP-70 is positively regulated by phosphorylation on Tyr319, which mediates the SH2-dependent interaction between Lck and ZAP-70 (5,6).
p-ZAP-70 Antibody (Tyr 319)-R Data
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sc-12946-R
p-ZAP-70 (Tyr 319)-R: sc-12946-R. Western blot analysis of ZAP-70 phosphoryation in Jurkat whole cell lysate.
sc-12946-R
Western blot analysis of ZAP-70 phosphorylation in untreated (A,D), pervanadate treated (B,E) and pervanadate and lambda protein phosphatase treated (C,F) Jurkat whole cell lysates. Antibodies tested include p-ZAP-70 (Tyr 319)-R: sc-12946-R (A,B,C) and ZAP-70 (LR): sc-574 (D,E,F).
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