p53 Antibody (DO-1): sc-126

p53 Antibody (DO-1) is an IgG2a kappa mouse monoclonal p53 antibody (also designated tumor suppressor protein p53 antibody or TP53 antibody) that detects the p53 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM. p53 Antibody (DO-1) is available as both the non-conjugated anti-p53 antibody form, as well as multiple conjugated forms of anti-p53 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The 53 protein is a DNA-binding, oligomerization domain- and transcription activation domain-containing tumor suppressor. The p53 protein upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation, and cell cycle control mechanisms. The p53 Antibody can stain both the nucleus and cytoplasm, as the p53 protein localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator, MDM2. MDM2 is an E3 ubiquitin ligase that is upregulated in the presence of active p53, where it poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active DNA-binding conformations and is differentially activated through posttranslational modifications, including phosphorylation and acetylation. phospho-specific p53 Antibodies can differentiate between phosphorylated and non-phosphorylated p53. Mutations in the DNA-binding domain (DBD) of p53, amino acids 110-286, can compromise energetically-favorable association with cis elements and are implicated in several human cancers. p53 Antibody is therefore an important reagent for cancer research.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

p53 Antibody (DO-1) References:

1. p53 website and analysis of p53 gene mutations in human cancer: forging a link between epidemiology and carcinogenesis.  |  Soussi, T., et al. 2000. Hum Mutat. 15: 105-13. PMID: 10612830
2. Regulation of p53 stability.  |  Ashcroft, M. and Vousden, KH. 1999. Oncogene. 18: 7637-43. PMID: 10618703
3. The role of tetramerization in p53 function.  |  Chène, P. 2001. Oncogene. 20: 2611-7. PMID: 11420672
4. Distinct pattern of p53 phosphorylation in human tumors.  |  Minamoto, T., et al. 2001. Oncogene. 20: 3341-7. PMID: 11423984
5. Regulation of the specific DNA binding function of p53.  |  Hupp, TR., et al. 1992. Cell. 71: 875-86. PMID: 1423635
6. Isolation of human-p53-specific monoclonal antibodies and their use in the studies of human p53 expression.  |  Banks, L., et al. 1986. Eur J Biochem. 159: 529-34. PMID: 2428616
7. Targeting mutant p53 for efficient cancer therapy.  |  Bykov, VJN., et al. 2018. Nat Rev Cancer. 18: 89-102. PMID: 29242642
8. Small-molecule modulation of p53 protein-protein interactions.  |  Kuusk, A., et al. 2020. Biol Chem. 401: 921-931. PMID: 32049643
9. A comprehensive study of p53 protein.  |  Patil, MR. and Bihari, A. 2022. J Cell Biochem. 123: 1891-1937. PMID: 36183376
10. Inhibition of p53 protein aggregation as a cancer treatment strategy.  |  Kwan, K., et al. 2023. Curr Opin Chem Biol. 72: 102230. PMID: 36436275
11. The role of the p53 protein in the apoptotic response.  |  Lane, DP., et al. 1994. Philos Trans R Soc Lond B Biol Sci. 345: 277-80. PMID: 7846126
12. p53, the cellular gatekeeper for growth and division.  |  Levine, AJ. 1997. Cell. 88: 323-31. PMID: 9039259

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p53 Antibody (DO-1) has 6,683 citations in a variety of scientific publications.

Primary antibodies like Anti-p53 Antibody (DO-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p53 Antibody (DO-1) Specifications

Alternate Product Name: TP53

Product Clone Name: DO-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 11-25

Epitope Target: p53

Epitope Species: human

Product Reactivity/Detection: wild type and mutant p53 under denaturing and non-denaturing conditions

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6,683 citations in various scientific publications

Available Conjugates/Forms for p53 Antibody (DO-1): AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, TRITC: Rhodamine, X: TransCruz Reagent