epitope mapping within an internal region of β-1,4-GalNAc-T2 of human origin
recommended for detection of β-1,4-GalNAc-T2 of mouse, rat and human origin by WB, IF and ELISA; non cross-reactive with family members β-1,4-GalNAc-T, β-1,4-GalNAc-T3, or β-1,4-GalNAc-T4; also reactive with additional species, including equine, canine, bovine and porcine
β-1,4-GalNAc-T2 Background Information Beta-1,4-N-acetyl-galactosaminyl transferase 2 (∫-1,4-GalNAc-T2) is a 566 amino acid protein belonging to the glycosyltransferase 2 family. Localized to the membrane of the Golgi apparatus, ∫-1,4-GalNAc-T2 participates in the synthesis of the Sd(a) antigen, a carbohydrate determinant expressed on erythrocytes, colonic mucosa and other tissues. During Sd(a) production, ∫-1,4-GalNAc-T2 transfers a beta-1,4-linked GalNAc to the galactose residue of an alpha-2,3-sialylated chain. ∫-1,4-GalNAc-T2 also catalyzes the last step in the biosynthesis of the Cad antigen. ∫-1,4-GalNAc-T2 is widely expressed, with the highest expression in colon and lesser expression in kidney, stomach, ileum and rectum. Mutations in the gene encoding ∫-1,4-GalNAc-T2 have been linked to Type I von Willebrand disease (VWD), the most common bleeding disorder in humans, characterized by reduced levels of plasma von Willebrand factor. Two named isoforms of ∫-1,4-GalNAc-T2 exist as a result of alternative splicing events.
