Rb Antibody (IF8): sc-102

Rb Antibody (IF8) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Rb protein (also called retinoblastoma (RB) transcriptional corepressor 1 or RB1) of mouse, rat and human origin by western blotting, immunoprecipitation, immunofluorescence, immunohistochemistry on paraffin embedded sections, and flow cytometry. Rb monoclonal antibody (IF8) is available as both non-conjugated form, as well as multiple conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. Pediatric cancer retinoblastoma and formation of other human tumors can be attributed to mutations in the retinoblastoma tumor suppressor gene. Rb protein regulates differentiation, apoptosis and cell cycle control by coordinating the cell cycle, at G1/S, with transcriptional machinery that includes the heterodimeric E2F family. During G1, cyclin D (D1, D2, D3)-dependent kinase-mediated phosphorylation of Rb at Ser 795 marks the conversion of Rb from a transcriptionally repressive, hypophosphorylated state to an inactive, phosphorylated state, which may be sustained through mitosis by differential phosphorylation of up to 16 putative serine or threonine residues, including Ser 249/Thr 252, Thr 373, Thr 356, Ser 780, Ser 807/Ser 811 and Thr 821/Thr 826. Hypophosphorylated Rb represses transcription of genes controlling cell cycle through direct protein-protein interactions, by binding and inactivating promoters of transcription factors, and through recruitment of histone deacetylase, which deacetylates promoter regions and enhances nucleosome formation, thereby masking transcription enhancing cis elements. Anti-Rb monoclonal antibody (IF8) is recommended for researchers investigating cell cycle regulation and molecular mechanisms underlying cancer development.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Rb Antibody (IF8) References:

1. Discovery of a regulatory motif that controls the exposure of specific upstream cyclin-dependent kinase sites that determine both conformation and growth suppressing activity of pRb.  |  Driscoll, B., et al. 1999. J Biol Chem. 274: 9463-71. PMID: 10092628
2. Glutamic acid mutagenesis of retinoblastoma protein phosphorylation sites has diverse effects on function.  |  Barrientes, S., et al. 2000. Oncogene. 19: 562-70. PMID: 10698526
3. Transforming growth factor beta inhibits the phosphorylation of pRB at multiple serine/threonine sites and differentially regulates the formation of pRB family-E2F complexes in human myeloid leukemia cells.  |  Hu, X., et al. 2000. Biochem Biophys Res Commun. 276: 930-9. PMID: 11027571
4. The role of the retinoblastoma protein (Rb) in the nuclear localization of BAG-1: implications for colorectal tumour cell survival.  |  Clemo, NK., et al. 2005. Biochem Soc Trans. 33: 676-8. PMID: 16042572
5. Retinoblastoma protein (RB) interacts with E2F3 to control terminal differentiation of Sertoli cells.  |  Rotgers, E., et al. 2014. Cell Death Dis. 5: e1274. PMID: 24901045
6. Phosphorylation of the Retinoblastoma protein (Rb) on serine-807 is required for association with Bax.  |  Antonucci, LA., et al. 2014. Cell Cycle. 13: 3611-7. PMID: 25483096
7. Retinoblastoma protein as an intrinsic BRD4 inhibitor modulates small molecule BET inhibitor sensitivity in cancer.  |  Ding, D., et al. 2022. Nat Commun. 13: 6311. PMID: 36274096
8. The retinoblastoma protein and cell cycle control.  |  Weinberg, RA. 1995. Cell. 81: 323-30. PMID: 7736585
9. Direct transcriptional repression by pRB and its reversal by specific cyclins.  |  Bremner, R., et al. 1995. Mol Cell Biol. 15: 3256-65. PMID: 7760821
10. Cancer cell cycles.  |  Sherr, CJ. 1996. Science. 274: 1672-7. PMID: 8939849
11. Cyclin D1/Cdk4 regulates retinoblastoma protein-mediated cell cycle arrest by site-specific phosphorylation.  |  Connell-Crowley, L., et al. 1997. Mol Biol Cell. 8: 287-301. PMID: 9190208
12. Rb interacts with histone deacetylase to repress transcription.  |  Luo, RX., et al. 1998. Cell. 92: 463-73. PMID: 9491888

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Rb Antibody (IF8) has 388 citations in a variety of scientific publications.

Primary antibodies like Anti-Rb Antibody (IF8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Rb Antibody (IF8) Specifications

Alternate Product Name: RB1

Product Clone Name: IF8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 703-722

Epitope Target: Rb

Epitope Species:

Product Reactivity/Detection: Rb p110

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 388 citations in various scientific publications

Available Conjugates/Forms for Rb Antibody (IF8): AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, TRITC: Rhodamine