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p-HSP 27 Anticuerpo(Ser 82): sc-101700

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(Basado en el análisis de datos)

  • p-HSP 27 Antibody (Ser 82) is a rabbit polyclonal IgG provided at 100 µg/ml
  • epitope corresponding to phosphorylated Ser 82 of HSP 27 of human origin
  • recommended for detection of Ser 82 phosphorylated HSP 27 of mouse and human origin by WB, IP, IF and IHC(P)
 

Ver tambiénHSP AntibodiesincluyebdoHSP, HSP 78, HSP 10, HSP 20, HSP 27, HSP 40, HSP 47, HSP 56, HSP 60, HSP 65, HSC 70, HSP 70, HSP 75, HSP 90 alpha, HSP 90 beta, Hsp90, Hsp104yHSP 105.

También verHSP 27 Inhibitors for functional analysis of cellular responses to HSP 27.


Información sobre pedidosInformación del gen
Productos Complementarios Recomendados:
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WB   IP   IF   IHC(P)   siRNA  
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 Información sobre pedidos
Nombre del productoNúmero de catálogoUnidadPrecioCantidadAñadir 
p-HSP 27 Antibody (Ser 82) sc-101700 100 µg/ml $279
Lambda Phosphatase sc-200312 100000 units in 0.25 ml $462
Lambda Phosphatase sc-200312A 20000 units in 0.05 ml $116
Lambda Phosphatase sc-200312 100000 units in 0.25 ml $462
Lambda Phosphatase sc-200312A 20000 units in 0.05 ml $116
 Silenciadores de Genes siRNA (Haga click en el nombre del producto para obtener más información)
Nombre del productoNúmero de catálogoUnidadPrecioCantidadAñadir 
HSP 27 siRNA (h) sc-29350 10 µM $258
HSP 27 siRNA (m) sc-35598 10 µM $258
HSP 27 (h)-PR sc-29350-PR 10 µM, 20 µl $23
HSP 27 (m)-PR sc-35598-PR 10 µM, 20 µl $23
 Plásmidos shRNA (Haga click en el nombre del producto para obtener más información)
Nombre del productoNúmero de catálogoUnidadPrecioCantidadAñadir 
HSP 27 shRNA Plasmid (h) sc-29350-SH 20 µg $520
HSP 27 shRNA Plasmid (m) sc-35598-SH 20 µg $520
 shRNA partículas antivirales (Haga click en el nombre del producto para obtener más información)
Nombre del productoNúmero de catálogoUnidadPrecioCantidadAñadir 
HSP 27 shRNA (h) Lentiviral Particles sc-29350-V 200 µl $625
HSP 27 shRNA (m) Lentiviral Particles sc-35598-V 200 µl $625
 Lisado de células para control positivo de WB (Haga click en el nombre del producto para obtener más información)
Nombre del productoNúmero de catálogoUnidadPrecioCantidadAñadir 
HeLa + heat shock Cell Lysate sc-2272 500 µg/200 µl $104
HeLa + UV Cell Lysate sc-2221 500 µg/200 µl $104
ECV304 Cell Lysate sc-2269 500 µg/200 µl $104
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
HSP 27 (h): 293T Lysate sc-174710 100 µg/200 µl $205
HSP 27 (h2): 293T Lysate sc-174711 100 µg/200 µl $205
HSP 27 (h4): 293T Lysate sc-174723 100 µg/200 µl $205
 
Especies Nombre del Gen Gene ID Ubicación en el cromosoma Numero de Acceso de Isoforma (ARNm) Número de Acceso de la Proteína Número OMIM™:
Humano HSPB1 3315 7q11.23 NM_001540 P04792
608634
Ratón Hspb1 15507 5 G2 NM_013560 P14602
No Disponible
 


HSP 27 is a constitutively expressed cytoplasmic protein that co-localizes to the nucleus upon stress-induced insult. Heat shock, cytokines and hormones are among the factors that stimulate the synthesis of HSP 27. The intracellular concentration of the mammalian heat shock protein, HSP 27, increases several-fold after heat shock and other metabolic stresses, and is closely associated with the acquisition of thermotolerance. MAP kinase-activated protein kinase-2 phosphorylates HSP 27 on Serine residues Ser 15, Ser 78 and Ser 82, which are phosphorylated in vivo in response to growth factors and heat shock. Ser 15, Ser 78 and Ser 82 occur in the sequence motif RXXS, which is recognized by ribosomal protein S6 kinase II.

p-HSP 27 Antibody (Ser 82) Data
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p-HSP 27 (Ser 82): sc-101700. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic localization.
p-HSP 27 (Ser 82): sc-101700. Western blot analysis of phosphorylated HSP 27 expression in untreated (A), UV-treated (B) and Heat Shock-treated (C) HeLa whole cell lysates.
p-HSP 27 (Ser 82): sc-101700. Western blot analysis of HSP 27 phosphorylation in untreated (A), UV irradiated (B), heat shocked (C), lambda protein phosphatase treated (D), UV irradiated and lambda protein phosphatase treated (E) and heat shocked and lambda protein phosphatase treated (F) HeLa whole cell lysate.
p-HSP 27 (Ser 82): sc-101700. Western blot analysis of HSP 27 phosphorylation in non-transfected 293T: sc-117752 (A), mouse HSP 27 transfected 293T: sc-120910 (B) and ECV304 (C) whole cell lysates.
Western blot analysis of HSP 27 phosphorylation in untreated (A, F), UV irradiated (B, G), heat shocked (C, H), UV irradiated and lambda protein phosphatase treated (D, I) and heat shocked and lambda protein phosphatase treated (E, J) HeLa whole cell lysates. Antibodies tested include p-HSP 27 (Ser 82): sc-101700 (A, B, C) and HSP 27 (M-20): sc-1049 (D, E, F).
Western blot analysis of HSP 27 phosphorylation in untreated (A,D), UV irradiated (B,E) and UV irradiated and lambda protein phosphatase treated (C,F) HeLa whole cell lysates. Antibodies tested include p-HSP 27 (Ser 82): sc-101700 (A,B,C) and HSP 27 (F-4): sc-13132 (D,E,F).
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