KiSS-1 Antibody (24-Q): sc-101246

KiSS-1 Antibody (24-Q) is a mouse monoclonal IgG2a kappa light chain antibody that detects KiSS-1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). KiSS-1 (24-Q) antibody is available as a non-conjugated format. KiSS-1, consisting of 145 amino acids, plays a crucial role in suppressing the metastasis of melanomas and breast carcinomas while leaving tumorigenicity unaffected, making KiSS-1 an important target for cancer research. The human KiSS-1 gene is located on chromosome 1q32 and is primarily expressed in the brain and placenta, highlighting KiSS-1′s potential involvement in neuroendocrine signaling and reproductive functions. Notably, KiSS-1 contains a polyproline-rich domain that serves as a ligand for SH3 domains and features a putative phosphorylation site for protein kinase C-alpha, which may influence KiSS-1′s regulatory functions in cellular signaling pathways. Additionally, KiSS-1 has been shown to reduce the binding of NFkappaB p50/p65 to the MMP-9 promoter, correlating with decreased expression of MMP-9, a key player in extracellular matrix remodeling and cancer progression. Furthermore, KiSS-1 exhibits agonist activity on the orphan G protein-coupled receptor GPR54, indicating KiSS-1′s potential role in mediating various physiological responses.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

KiSS-1 Antibody (24-Q) References:

1. KiSS-1 represses 92-kDa type IV collagenase expression by down-regulating NF-kappa B binding to the promoter as a consequence of Ikappa Balpha -induced block of p65/p50 nuclear translocation.  |  Yan, C., et al. 2001. J Biol Chem. 276: 1164-72. PMID: 11060311
2. AXOR12, a novel human G protein-coupled receptor, activated by the peptide KiSS-1.  |  Muir, AI., et al. 2001. J Biol Chem. 276: 28969-75. PMID: 11387329
3. The metastasis suppressor gene KiSS-1 encodes kisspeptins, the natural ligands of the orphan G protein-coupled receptor GPR54.  |  Kotani, M., et al. 2001. J Biol Chem. 276: 34631-6. PMID: 11457843
4. The Kiss-1/Kiss-1R complex as a negative regulator of cell motility and cancer metastasis (Review).  |  Ji, K., et al. 2013. Int J Mol Med. 32: 747-54. PMID: 23969598
5. GLP-1 increases Kiss-1 mRNA expression in kisspeptin-expressing neuronal cells.  |  Oride, A., et al. 2017. Biol Reprod. 97: 240-248. PMID: 29044434
6. KISS1 in breast cancer progression and autophagy.  |  Ulasov, IV., et al. 2019. Cancer Metastasis Rev. 38: 493-506. PMID: 31705228
7. Placental protein expression of kisspeptin-1 (KISS1) and the kisspeptin-1 receptor (KISS1R) in pregnancy complicated by diabetes mellitus or preeclampsia.  |  Kapustin, RV., et al. 2020. Arch Gynecol Obstet. 301: 437-445. PMID: 31811415
8. The KiSS-1/GPR54 system: Essential roles in physiological homeostasis and cancer biology.  |  Zhu, N., et al. 2022. Genes Dis. 9: 28-40. PMID: 35005105
9. KISS-1 knockdown inhibits cell growth, migration, and invasion in HTR-8/SVneo cells by regulating the GRP54-mediated PI3K/AKT signaling pathway.  |  Chen, L., et al. 2024. Autoimmunity. 57: 2297564. PMID: 38155490
10. KiSS-1, a novel human malignant melanoma metastasis-suppressor gene.  |  Lee, JH., et al. 1996. J Natl Cancer Inst. 88: 1731-7. PMID: 8944003
11. Suppression of metastasis in human breast carcinoma MDA-MB-435 cells after transfection with the metastasis suppressor gene, KiSS-1.  |  Lee, JH. and Welch, DR. 1997. Cancer Res. 57: 2384-7. PMID: 9192814
12. Chromosome localization and genomic structure of the KiSS-1 metastasis suppressor gene (KISS1).  |  West, A., et al. 1998. Genomics. 54: 145-8. PMID: 9806840

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-KiSS-1 Antibody (24-Q) has 21 citations in a variety of scientific publications.

Primary antibodies like Anti-KiSS-1 Antibody (24-Q) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

KiSS-1 Antibody (24-Q) Specifications

Alternate Product Name: KiSS-1

Product Clone Name: 24-Q

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 46-146

Epitope Target: KiSS-1

Epitope Species: human

Product Reactivity/Detection: KiSS-1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 21 citations in various scientific publications

Available Conjugates/Forms for KiSS-1 Antibody (24-Q): N/A