TAF II p55 Antibody (SQ-8): sc-101167

TAF II p55 Antibody (SQ-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects TAF II p55 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-TAF II p55 antibody (SQ-8) is available as the non-conjugated format. TAF II p55, also known as Transcription initiation factor TFIID 55 kDa subunit, TAF7, or TAF2F, is a crucial 349 amino acid component of the TFIID complex, which plays a vital role in the regulation of gene expression. TAF II p55 is primarily localized in the nucleus, where TAF II p55 is involved in the assembly of the preinitiation complex by interacting with the TATA-binding protein and other TBP-associated factors. TAF II p55 interacts with TAF II p250, the largest subunit of the TFIID complex, and several other proteins essential for transcriptional activation. Through these interactions, TAF II p55 functions as a regulatory checkpoint, inhibiting the acetyltransferase activity of binding partners, which delays transcription until the preinitiation complex is fully assembled. This regulatory mechanism ensures gene expression occurs in a controlled manner, preventing premature transcription that could lead to cellular dysfunction.

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TAF II p55 Antibody (SQ-8) References:

1. The intronless and TATA-less human TAF(II)55 gene contains a functional initiator and a downstream promoter element.  |  Zhou, T. and Chiang, CM. 2001. J Biol Chem. 276: 25503-11. PMID: 11340078
2. TAFII55 binding to TAFII250 inhibits its acetyltransferase activity.  |  Gegonne, A., et al. 2001. Proc Natl Acad Sci U S A. 98: 12432-7. PMID: 11592977
3. TAF7 (TAFII55) plays a role in the transcription activation by c-Jun.  |  Munz, C., et al. 2003. J Biol Chem. 278: 21510-6. PMID: 12676957
4. TATA-binding protein-associated factor 7 regulates polyamine transport activity and polyamine analog-induced apoptosis.  |  Fukuchi, J., et al. 2004. J Biol Chem. 279: 29921-9. PMID: 15078871
5. TAF7: a possible transcription initiation check-point regulator.  |  Gegonne, A., et al. 2006. Proc Natl Acad Sci U S A. 103: 602-7. PMID: 16407123
6. Presence of the anti-leukemic nucleotide analog, 2-chloro-2'-deoxyadenosine-5'-monophosphate, in a promoter sequence alters DNA binding of TATA-binding protein (TBP).  |  Hartman, WR., et al. 2007. Arch Biochem Biophys. 459: 223-32. PMID: 17320040
7. Contiguous X-chromosome deletion syndrome encompassing the BTK, TIMM8A, TAF7L, and DRP2 genes.  |  Sedivá, A., et al. 2007. J Clin Immunol. 27: 640-6. PMID: 17851739
8. Cloning of an intrinsic human TFIID subunit that interacts with multiple transcriptional activators.  |  Chiang, CM. and Roeder, RG. 1995. Science. 267: 531-6. PMID: 7824954
9. Multiple interactions between hTAFII55 and other TFIID subunits. Requirements for the formation of stable ternary complexes between hTAFII55 and the TATA-binding protein.  |  Lavigne, AC., et al. 1996. J Biol Chem. 271: 19774-80. PMID: 8702684

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TAF II p55 Antibody (SQ-8) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-TAF II p55 Antibody (SQ-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TAF II p55 Antibody (SQ-8) Specifications

Alternate Product Name: TAF II p55

Product Clone Name: SQ-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 130-224

Epitope Target: TAF II p55

Epitope Species: human

Product Reactivity/Detection: TAF II p55

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for TAF II p55 Antibody (SQ-8): N/A