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- mouse monoclonal IgG2a; 200 µg/ml
- raised against amino acids 1-238 representing full length GFP (green fluorescent protein) of Aequorea victoria origin
- recommended for detection of GFP and GFP mutant fusion proteins by WB, IP, IF, FCM and ELISA
- agarose conjugate for IP studies, sc-9996 AC, 500 µg/0.25 ml agarose
- HRP conjugate, sc-9996 HRP, 200 µg/ml
- biotin conjugate, sc-9996 B, 200 µg/ml
- fluorescein (sc-9996 FITC) and rhodamine (sc-9996 TRITC) conjugates for immunofluorescence, 200 µg/ml
- Alexa Fluor® 405 (sc-9996 AF405), Alexa Fluor® 488 (sc-9996 AF488) and Alexa Fluor® 647 (sc-9996 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
- phycoerythrin (sc-9996 PE), PerCP (sc-9996 PerCP) and PerCP-Cy5.5 (sc-9996 PCPC5) conjugates for FCM, 100 tests
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GFP Background Information
The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.
| GFP (B-2) Product Citations |
See how others have used GFP (B-2): sc-9996 antibody and or GFP (B-2) antibody conjugates.
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GFP (B-2)
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GFP (B-2): sc-9996. Western blot analysis of GFP expression in COS (A) and GFP transfected COS (B) cell lysates.
GFP (B-2): sc-9996. Immunofluorescence staining of methanol-fixed COS cells transfected with GFP fusion protein showing cytoplasmic staining.
pCruz GFP-Lac Z: sc-5046. Western blot analysis of GFP expression in control COS cells (A) and COS cells transfected with GFP-Lac Z (B). Blot probed with GFP (B-2): sc-9996. |
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