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- mouse monoclonal IgG1; 200 µg/ml
- raised against full length eIF4E of human origin
- recommended for detection of eIF4E of mouse, rat, human and porcine origin by WB, IP, IF, IHC(P) and ELISA
- agarose conjugate for IP studies, sc-9976 AC, 500 µg/0.25 ml agarose
- HRP conjugate, sc-9976 HRP, 200 µg/ml
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Ordering InformationProduct Citations
Recommended Support Products:
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| Species |
Gene Name |
Gene ID |
Chromosome Location |
Isoform (mRNA) Accession # |
Protein Accession # |
OMIM™ Number |
| Human |
EIF4E |
1977 |
4q23 |
NM_001968 |
P06730
|
133440 |
| Mouse |
Eif4e |
13684 |
3 G3 |
|
P63073
|
N/A |
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eIF4E Background Information
The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF4F exists in vitro as a trimeric complex of eIF4G, eIF4E, and eIF4A. Together, the complex allows ribosome binding to mRNA by inducing the unwinding of mRNA secondary structures (1,2). eIF4E binds to the mRNA “cap” during an early step in the initiation of protein synthesis (3,4). eIF4A acts as an ATP-dependent RNA helicase (5,6). eIF4G acts as a bridge between eIF4E, eIF4A, and the eIF3 complex (7,8).
| eIF4E (P-2) Product Citations |
See how others have used eIF4E (P-2): sc-9976 antibody and or eIF4E (P-2) antibody conjugates.
15 total citations Loading citations.
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eIF4E (P-2)
Click on image to enlarge
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eIF4E (P-2): sc-9976. Western blot analysis of eIF4E expression in KNRK (A) and K-562 (B) whole cell lysates.
Western blot detection of eIF4E phosphorylation in NIH/3T3 cells that were serum starved and then treated with serum. Blots were probed with eIF4E (P-2): sc-9976 (A) and p-eIF4E (Ser 209)-R: sc-12885-R (B,C). In B and C, the antibody was preincubated with cognate non-phosphorylated or phosphorylated peptide, respectively. eIF4E (P-2): sc-9976. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human pancreas tumor (A) and immunofluorescence staining of methanol-fixed KNRK cells (B) showing cytoplasmic staining.
eIF4E (P-2): sc-9976. Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing cytoplasmic staining of squamous epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
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