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- goat polyclonal IgG, 200 µg/ml; also available as rabbit IgG, 200 µg/ml, sc-9857-R
- epitope mapping at the C-terminus of PCNA of human origin
- recommended for detection of PCNA of mouse, rat, human and Xenopus origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
- agarose conjugate for IP studies, sc-9857 AC, 500 µg/0.25 ml agarose
- blocking peptide, sc-9857 P
- fluorescein (sc-9857 FITC) and rhodamine (sc-9857 TRITC) conjugates for immunofluorescence, 200 µg/ml
- Alexa Fluor® 405 (sc-9857 AF405), Alexa Fluor® 488 (sc-9857AF488) and Alexa Fluor® 647 (sc-9857 AF647) conjugates for immunofluorescence; 100 µg/2 ml
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PCNA Background Information
The proliferating cell nuclear antigen (PCNA), a protein synthesized in early G1 and S phases of the cell cycle, functions in cell cycle progression, DNA replication and DNA repair. In early S phase, PCNA exhibits granular distribution and is absent from the nucleoli; however, in late S phase, it relocates to the nucleoli. PCNA exists in two basic forms: one involved in ongoing DNA replication, which localizes specifically to the nucleus, and a second, soluble form, not implicated in constant synthesis. Interestingly, the latter form degrades in the presence of organic solvents, rendering it undetectable by histological methods in tissues using organic fixatives, and thus also providing a method of visualizing only the synthesizing form.
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See how others have used PCNA (C-20): sc-9857 antibody and or PCNA (C-20) antibody conjugates.
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PCNA (C-20)
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PCNA (C-20): sc-9857. Immunofluorescence staining of methanol-fixed A-431 cells showing nuclear staining.
PCNA (C-20): sc-9857. Western blot analysis of PCNA expression in A-431 (A) and NIH/3T3 (B) whole cell lysates.
PCNA (C-20): sc-9857. Western blot analysis of PCNA expression in Hep G2 whole cell lysate (A) and mouse liver tissue extract (B).
PCNA (C-20): sc-9857. Immunofluorescence staining of methanol-fixed A-431 cells showing nuclear localization using indirect FITC (A) staining and HeLa cells using direct Alexa Fluor 488 (B) staining.
PCNA (C-20): sc-9857. Western blot analysis of PCNA expression in non-transfected 293T: sc-117752 (A), mouse PCNA transfected 293T: sc-127307 (B) and A-431 (C) whole cell lysates. PCNA (C-20)-R: sc-9857-R. Western blot analysis of PCNA expression in non-transfected 293T: sc-117752 (A), mouse PCNA transfected 293T: sc-127307 (B) and A-431 (C) whole cell lysates. PCNA (C-20): sc-9857. Western blot analysis of PCNA expression in non-transfected: sc-117752 (A) and human PCNA transfected: sc-159920 (B) 293T whole cell lysates. PCNA (C-20)-R: sc-9857-R. Western blot analysis of PCNA expression in non-transfected: sc-117752 (A) and human PCNA transfected: sc-159920 (B) 293T whole cell lysates. PCNA (C-20)-R: sc-9857-R. Western blot analysis of PCNA expression in non-transfected: sc-117752 (A) and human PCNA transfected: sc-170834 (B) 293T whole cell lysates. PCNA (C-20): sc-9857. Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear staining of cells in seminiferous ducts.
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