PML Antibody (PG-M3): sc-966

PML Antibody (PG-M3) is a mouse monoclonal IgG1 kappa light chain antibody that detects PML protein of human origin through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry (IHC). PML (PG-M3) monoclonal antibody is offered in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. PML functions as a zinc finger transcription factor and is crucial for regulating gene expression involved in cell differentiation and apoptosis. PML′s role in these processes is particularly significant in acute promyelocytic leukemia, where PML gene undergoes a chromosomal translocation with the retinoic acid receptor α (RARα) gene, resulting in the formation of the PML/RARα chimeric protein. This aberrant protein disrupts normal myeloid differentiation by recruiting the nuclear co-repressor-histone deacetylase complex, which is essential for retinoic acid-dependent differentiation, and also induces gene hypermethylation, leading to the silencing of critical genes and promoting leukemogenesis. PML′s ability to influence these pathways underscores PML′s importance in both normal cellular function and disease states, making PML (PG-M3) antibody a valuable tool for researchers studying these mechanisms.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

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PML Antibody (PG-M3) References:

1. Methyltransferase recruitment and DNA hypermethylation of target promoters by an oncogenic transcription factor.  |  Di Croce, L., et al. 2002. Science. 295: 1079-82. PMID: 11834837
2. Identification of DNA rearrangements at the retinoic acid receptor-alpha (RAR-alpha) locus in all patients with acute promyelocytic leukemia (APL) and mapping of APL breakpoints within the RAR-alpha second intron. Italian Cooperative Study Group 'GIMEMA'.  |  Diverio, D., et al. 1992. Blood. 79: 3331-6. PMID: 1317727
3. Structure and origin of the acute promyelocytic leukemia myl/RAR alpha cDNA and characterization of its retinoid-binding and transactivation properties.  |  Pandolfi, PP., et al. 1991. Oncogene. 6: 1285-92. PMID: 1650447
4. Chromosomal translocation t(15;17) in human acute promyelocytic leukemia fuses RAR alpha with a novel putative transcription factor, PML.  |  Kakizuka, A., et al. 1991. Cell. 66: 663-74. PMID: 1652368
5. Characterization of a zinc finger gene disrupted by the t(15;17) in acute promyelocytic leukemia.  |  Goddard, AD., et al. 1991. Science. 254: 1371-4. PMID: 1720570
6. The t(15;17) translocation of acute promyelocytic leukaemia fuses the retinoic acid receptor alpha gene to a novel transcribed locus.  |  de Thé, H., et al. 1990. Nature. 347: 558-61. PMID: 2170850
7. Molecular analysis of acute promyelocytic leukemia breakpoint cluster region on chromosome 17.  |  Borrow, J., et al. 1990. Science. 249: 1577-80. PMID: 2218500
8. Promyelocytic leukemia (PML) protein plays important roles in regulating cell adhesion, morphology, proliferation and migration.  |  Tang, MK., et al. 2013. PLoS One. 8: e59477. PMID: 23555679
9. PML protein organizes heterochromatin domains where it regulates histone H3.3 deposition by ATRX/DAXX.  |  Delbarre, E., et al. 2017. Genome Res. 27: 913-921. PMID: 28341773
10. Phospho-SIM and exon8b of PML protein regulate formation of doxorubicin-induced rDNA-PML compartment.  |  Hornofova, T., et al. 2022. DNA Repair (Amst). 114: 103319. PMID: 35325646
11. The acute promyelocytic leukemia-specific PML-RAR alpha fusion protein inhibits differentiation and promotes survival of myeloid precursor cells.  |  Grignani, F., et al. 1993. Cell. 74: 423-31. PMID: 8394219
12. Fusion proteins of the retinoic acid receptor-alpha recruit histone deacetylase in promyelocytic leukaemia.  |  Grignani, F., et al. 1998. Nature. 391: 815-8. PMID: 9486655

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PML Antibody (PG-M3) has 709 citations in a variety of scientific publications.

Primary antibodies like Anti-PML Antibody (PG-M3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PML Antibody (PG-M3) Specifications

Alternate Product Name: PML

Product Clone Name: PG-M3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 37-51

Epitope Target: PML

Epitope Species: human

Product Reactivity/Detection: all isoforms of PML

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 709 citations in various scientific publications

Available Conjugates/Forms for PML Antibody (PG-M3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, TRITC: Rhodamine, X: TransCruz Reagent