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- mouse monoclonal IgG1; 200 µg/ml
- N-terminal epitope corresponding to residues 37-51 of PML of human origin
- recommended for detection of all isoforms of PML of human origin by WB, IP, IF and IHC(P)
- TransCruz reagent for Gel Supershift and ChIP applications, PML (PG-M3) X: sc-966 X, 200 µg/0.1 ml
- fluorescein (PML (PG-M3) FITC: sc-966 FITC) and rhodamine (PML (PG-M3) TRITC: sc-966 TRITC) conjugates for immunofluorescence, 200 µg/ml
- Alexa Fluor® 405 (PML (PG-M3) Alexa Fluor® 405: sc-966 AF405), Alexa Fluor® 488 (PML (PG-M3) Alexa Fluor® 488: sc-966 AF488) and Alexa Fluor® 647 (PML (PG-M3) Alexa Fluor® 647: sc-966 AF647) conjugates for immunofluorescence; 100 µg/2 ml
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PML Background Information
The PML protein is a zinc finger transcription factor expressed as three major transcription products due to alternative splicing. The gene encoding human PML maps to chromosome 15q22. The t(15;17) (q22;q11.2-q12) chromosomal translocation of the retinoic acid receptor å (RARå) gene occurs in virtually all cases of acute promyelocytic leukemia and results in the expression of a PML/RARa chimeric protein. Myeloid precursor cells expressing the PML/RARå chimera fail to differentiate and exhibit an increased growth rate consequent to diminished apoptosis. PML/RARå transforms myeloid precursors by recruiting the nuclear co-repressor (N-CoR)-histone deacetylase complex that is essential to retinoic acid-dependent myeloid differentiation. PML/RARå also recruits DNA methyltransferases in order to induce gene hypermethylation and silencing, which ultimately facilitates leukemogenesis.
| PML (PG-M3) Product Citations |
See how others have used PML (PG-M3): sc-966 antibody and or PML (PG-M3) antibody conjugates.
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PML (PG-M3)
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PML (PG-M3): sc-966. Western blot analysis of PML expression in K-562 (A) and COLO 320DM (B) whole cell lysates.
PML (PG-M3): sc-966. Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human lymph node showing nuclear staining (A). Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear staining (B).
PML (PG-M3): sc-966. Immunoperoxidase staining of formalin fixed, paraffin-embedded human lymph node tissue showing nuclear staining of lymphoid cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
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