PAL (N-17) Antibody: sc-9536

PAL Background Information
The Src homology 3 (SH3) region is a small protein domain of approximately 60 amino acids present in a large group of proteins (1,2). In general, it exists in association with catalytic domains, as in the nonreceptor protein-tyrosine kinases and phospholipase C-g, within structural proteins, such as spectrin or myosin, and in small adapter proteins, such as Crk and GRB2 (3-6). SH3 domains are often accompanied by SH2 domains of 100 amino acids that bind to tyrosine-phosphorylated regions of target proteins, frequently linking activated growth factors to putative signal transduction proteins (3-6). Deletion or mutation of SH3 domains generally activates the transforming potential of nonreceptor tyrosine kinases, suggesting that SH3 mediates negative regulation of an intrinsic transforming activity (2,3). PAL (protein expressed in activated lymphocytes) is an SH2 domain-binding adapter protein that is expressed in actively dividing and proliferating cells, suggesting a role for PAL in governing cell cycle progression (7).