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- rabbit polyclonal IgG, 200 µg/ml; also available as goat IgG, 200 µg/ml, sc-947-G
- epitope mapping at the N-terminus of TRAF3 of mouse origin
- recommended for detection of TRAF3 of mouse and rat origin by WB, IP, IF, IHC(P), FCM and ELISA
- blocking peptide, sc-947 P
- phycoerythrin conjugate for intracellular FCM, sc-947 PE, 100 tests
- Alexa Fluor® 405 (sc-947 AF405), Alexa Fluor® 488 (sc-947 AF488) and Alexa Fluor® 647 (sc-947 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
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Ordering InformationProduct Citations
Recommended Support Products:
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| Species |
Gene Name |
Gene ID |
Chromosome Location |
Isoform (mRNA) Accession # |
Protein Accession # |
OMIM™ Number |
| Mouse |
Traf3 |
22031 |
12 F1 |
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Q60803
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N/A |
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TRAF3 Background Information
Tumor necrosis factor (TNF)-activated cell signaling is mediated primarily through the TNF receptor 1 (TNF-R1) and, to a lesser extent, TNF-R2. Both TNF receptors are members of the expanding TNF receptor superfamily which includes the FAS antigen and CD40. Potential insight into an understanding of TNF receptor-mediated signaling was provided by the identification of two related proteins, TRAF1 and TRAF2 (for TNF receptor-associated factors 1 and 2, respectively). Both function to form heterodimeric complexes and associate with the cytoplasmic domain of TNF-R2. A third member of this protein family, alternatively designated CD40 bp, CRAF1, LAP1 or TRAF3, has been identified and shown to associate with the cytoplasmic domain of CD40. The similarity between a specific region of TRAF3 with regions of TRAF1 and TRAF2 define a “TRAF-C” domain that is necessary and sufficient for CD40 binding and homodimerization.
| TRAF3 (M-20) Product Citations |
See how others have used TRAF3 (M-20): sc-947 antibody and or TRAF3 (M-20) antibody conjugates.
6 total citations Loading citations.
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TRAF3 (M-20)
Click on image to enlarge
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Immunofluorescence staining of methanol-fixed NIH/3T3 cells transfected with mouse TRAF3. Antibodies tested include TRAF3 (M-20): sc-947 (A), TRAF3 (C-20): sc-949 (B) and TRAF3 (M-51): sc-1574 (C). Western blot analysis of TRAF3 expression in TRAF3 transfected NIH/3T3 cells (A-C). Antibodies tested include TRAF3 (M-20): sc-947 (A), TRAF3 (C-20): sc-949 (B) and TRAF3 (M-51): sc-1574 (C). TRAF3 (M-20) PE: sc-947 PE. Intracellular FCM analysis of fixed and permeabilized control (green line histogram) and TRAF3 transfected (solid orange histogram) IB4 cells. Dotted pink histogram represents the isotype control, normal goat IgG: sc-3992. TRAF3 (M-20)-G: sc-947-G. Western blot analysis of TRAF3 expression in TRAF3 transfected (A) and control (B) NIH/3T3 cells. TRAF3 (M-20) Alexa Fluor 488: sc-947 AF488. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal rabbit IgG: sc-45068. TRAF3 (M-20) Alexa Fluor 647: sc-947 AF647. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal rabbit IgG: sc-24647. TRAF3 (M-20): sc-947. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization using indirect FITC (A) staining and HeLa cells using direct Alexa Fluor 488 (B) staining.
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