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NGEP siRNA (h), shRNA and Lentiviral Particle Gene Silencers

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See NGEP Antibodies for analysis of cellular responses to NGEP gene silencers, including NGEP siRNA, NGEP shRNA Plasmids and NGEP shRNA Lentiviral Particles.

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 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
NGEP siRNA (h) sc-94463 10 µM $258
NGEP (h)-PR sc-94463-PR 10 µM, 20 µl $23
NGEP shRNA Plasmid (h) sc-94463-SH 20 µg $520
NGEP shRNA (h) Lentiviral Particles sc-94463-V 200 µl $625
NGEP siRNA (h) : sc-94463  |  siRNA Datasheet / Protocol
  • 10 µM, 50-100 transfections
  • siRNA products generally consist of pools of three to five target-specific 19-25 nt siRNAs designed to knockdown gene expression
  • for independent verification of NGEP gene silencing results, individual siRNA duplex components are also available upon request
  • suitable control antibody: contact Technical Service for availability of control antibody
  • sqRT-PCR Primer also available: NGEP (h)-PR: sc-94463-PR, 20 µl
  • For further details describing the si/shRNA Gene Silencers click here
 
 Recommended siRNA Support Reagents (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
siRNA Dilution Buffer sc-29527 1.5 ml $5
siRNA Transfection Reagent sc-29528 0.3 ml $142
siRNA Transfection Medium sc-36868 20 ml $9
siRNA Reagent System sc-45064 1 kit $331
Control siRNA-A sc-37007 10 µM $77
Control siRNA (FITC Conjugate)-A sc-36869 10 µM $104
NGEP shRNA Plasmid (h): sc-94463-SH shRNA Datasheet / Protocol
  • 20 µg, up to 20 transfections
  • shRNA Plasmids generally consist of a pool of three to five lentiviral vector plasmids each encoding target-specific 19-25 nt (plus hairpin) shRNAs designed to knockdown gene expression
  • shRNA sequences correspond to NGEP siRNA Gene Silencer sequences
  • provided as transfection-ready purified plasmid DNA target-specific gene silencing
  • after transfection, cells stably expressing shRNAs can be isolated by puromycin selection
  • shRNA Plasmids are intended only for non-commercial use. Click here for details.
  • suitable control antibody: contact Technical Service for availability of control antibody
  • sqRT-PCR Primer also available: NGEP (h)-PR: sc-94463-PR, 20 µl
 Recommended shRNA Support Reagents (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Plasmid Transfection Reagent sc-108061 0.2 ml $71
Plasmid Transfection Medium sc-108062 20 ml $8
Control shRNA Plasmid-A sc-108060 20 µg $83
Control shRNA Plasmid-B sc-108065 20 µg $83
Control shRNA Plasmid-C sc-108066 20 µg $83
copGFP Control Plasmid sc-108083 20 µg $83
Puromycin dihydrochloride sc-108071 25 mg $30
 
NGEP shRNA (h)
Lentiviral Particles: sc-94463-V
Lentiviral Particles Datasheet / Protocol
  • 200 µl viral stock containing 1x 106 infectious units of virus (IFU) , sufficient for 10-20 transductions
  • Lentiviral Particles are provided as transduction-ready viral particles for gene silencing
  • Lentiviral Particles generally contain three to five expression constructs each encoding target-specific 19-25 nt (plus hairpin) shRNA designed to knockdown gene expression
  • shRNA sequences correspond to NGEP siRNA siRNA Gene Silencer sequences
  • After transduction, stable cell lines expressing the shRNA may be isolated via selection with puromycin
  • Biosafety - Lentiviral Particles are replication-incompetent and are designed to self-inactivate after transduction and integration of shRNA constructs into genomic DNA of target cells. Click here for details.
  • Lentiviral Particles are intended for non-commercial use. Click here for details.
 Recommended Lentiviral Particle Support Reagents (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Control shRNA Lentiviral Particles-A sc-108080 200 µl $176
copGFP Control Lentiviral Particles sc-108084 200 µl $176
Puromycin dihydrochloride sc-108071 25 mg $30
Polybrene® sc-134220 10 mg/ml $20
 
Recommended Support Products:
(click button of application of choice)
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human TMEM16G 50636 2q37.3 NM_001001666, NM_001001891 Q6IWH7
605096
 

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