FHL-5 Background Information The four-and-a-half-LIM domain (FHL) proteins include FHL-1 (SLIM1), FHL-2 (SLIM3), FHL-3 (SLIM2), FHL-4 and FHL-5. The signature “half-domain”, a single zinc finger domain located in the N-terminal region, differentiates FHLs from other LIM-only proteins, which have numbers of zinc fingers. Specific combinations of FHL proteins elicit selective activation of both CREB and CREM. FHL-5, also known as ACT (Activator of CREM in Testis), is a testis-specific protein that interacts with CREM (a transcription factor required for spermatid differentiation) via its third LIM domain and can stimulate CREM activity independently of phosphorylation. This suggests that FHL-5 may participate in the regulation of spermatogenesis by acting as a transcriptional coactivator of CREM. During spermatid elongation, FHL-5 is translocated from the nucleus to the cytoplasm by the kinesin motor protein KIF17 thus silencing CREM activity.
FHL-5 (K-18)
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FHL-5 (K-18): sc-9368. Western blot analysis of FHL-5 expression in RAW 264.7 nuclear extract.
