Insulin Antibody (H-86): sc-9168

Thank you for your question. This antibody has been discontinued. Please contact our technical service for information on this product.

Thank you for your question. This rabbit polyclonal antibody is now discontinued and no longer available for sale or distribution.

Thank you for your question. We recommend using a sodium citrate buffer (pH 6.0) with heat treatment for antigen retrieval with this antibody. A link to our complete recommended protocol for immunohistochemistry is provided below. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining

Thank you for your question. The Western blot data shown on the product datasheet for this primary antibody, Insulin (H-86): sc-9168, is against a human recombinant fusion protein corresponding to amino acids 22-110 of human insulin. This recombinant protein was developed by Santa Cruz Biotechnology.

Thank you for your question. It can be very difficult to detect endogenous insulin protein in cell lysates so we recommend you review the literature for recommended protocols. This antibody, sc-9168: Insulin (H-68), has been cited in the literature for use in Western blot many times and you can find those product citations on our website. The expected molecular weight, under reducing conditions is ~ 12 kDa for the unprocessed protein. The insulin A and B chains are both predicted to be ~24 kDa. Please contact our Technical Support office for assistance using the antibody.