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home » Synthesis and Degradation » Ubiquitin Antibody / Ubiquitin Antibodies » Ub (FL-76): sc-9133

Ub (FL-76) Antibody: sc-9133

  |  Datasheet
  • rabbit polyclonal IgG, 200 µg/ml
  • epitope corresponding to amino acids 1-76 representing full length Ub of human origin
  • recommended for detection of ubiquitin and polyubiquitin of mulitple species by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
  • agarose conjugate for IP studies, sc-9133 AC, 500 µg/0.25 ml agarose
 
Additional Ubiquitin Antibodies ...
 
Ordering InformationProduct Citations
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   IHC(P)   siRNA  
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human RPS27A 6233 2p16.1 NM_002954 P62979
 191343
Mouse Uba52 22186 8 B3.3 P62984
 N/A
Mouse Ubb 22187 11 B2 NP_035794
 N/A
Mouse Ubc 22190 5 G1.1 NP_062613
 N/A
Mouse Rps27a 78294 11 A3.3 P62983,P62991
 N/A
 
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 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
Ub (FL-76) sc-9133 200 µg/ml $279
Ub (FL-76) AC sc-9133 AC 500µg/ml, 25%ag $366
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Ub siRNA (h) sc-29513 10 µM $258
Ub siRNA (m) sc-36770 10 µM $258
Ub (h)-PR sc-29513-PR 10 µM $23
Ub (m)-PR sc-36770-PR 10 µM $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Ub shRNA Plasmid (h) sc-29513-SH 20 µg $520
Ub shRNA Plasmid (m) sc-36770-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Ub shRNA (h) Lentiviral Particles sc-29513-V 200 µl $625
Ub shRNA (m) Lentiviral Particles sc-36770-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Ub (h): 293T Lysate sc-111402 100µg/200µl $205
Jurkat Whole Cell Lysate sc-2204 500 µg/200 µl $104
NIH/3T3 Whole Cell Lysate sc-2210 500 µg/200 µl $104
MCF7 Whole Cell Lysate sc-2206 500 µg/200 µl $104
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
Jurkat + LLnL Cell Lysate sc-24715 500 µg/200 µl $104
UBC (m3): 293T Lysate sc-124400 100µg/200µl $205

Ub Background Information
Ubiquitin (Ub) is among the most phylogenetically conserved proteins known. The primary function of ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome. This small, 76 amino acid protein can be covalently attached to cellular proteins via an isopeptide linkage between the carboxy terminal group of ubiquitin and lysine amino groups on the acceptor protein. For proteolysis to occur, ubiquitin oligomers must be assembled. Ubiquitin chains on proteolytic substrates are commonly found to have an isopeptide bridge between Lysine 48 of one ubiquitin molecule and the carboxy-terminus of a neighboring ubiquitin molecule. Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IkBa and p27.

Ub (FL-76) Product Citations
See how others have used Ub (FL-76): sc-9133 antibody and or Ub (FL-76) antibody conjugates.


64 total citations
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Ub (FL-76)
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sc-9133
Ub (FL-76): sc-9133. Western blot analysis of lysates from control Jurkat cells (A), Jurkat cells treated with the proteasome inhibitor Lactacystin (sc-3575) showing accumulation of ubiquitinated proteins (B) and human recombinant Ub (C).
sc-9133
Ub (FL-76): sc-9133. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing cytoplasmic and nuclear staining.
sc-9133
Ub (FL-76): sc-9133. Western blot analysis of Ub expression in NIH/3T3 (A) and HeLa (B) whole cell lysates.
sc-9133
Ub (FL-76): sc-9133. Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing nuclear staining in decidual cells and cytoplasmic and nuclear stainining in trophoblastic cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
sc-9133
Ub (FL-76): sc-9133. Western blot analysis of Ub expression in non-transfected: sc-110760 (A) and human Ub transfected: sc-111402 (B) 293 whole cell lysates.
sc-9133
Ub (FL-76): sc-9133. Western blot analysis of Ub expression in non-transfected: sc-117752 (A) and human Ub transfected: sc-111402 (B) 293T whole cell lysates.
sc-9133
Ub (FL-76): sc-9133. Western blot analysis of Ub expression in 293T whole cell lysate.
sc-9133
Ub (FL-76): sc-9133. Western blot analysis of UBC expression in non-transfected: sc-117752 (A) and mouse UBC transfected: sc-124400 (B) 293T whole cell lysates.
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