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NCoA-3 Antibody (M-397): sc-9119

 |  Datasheet

(Based on data analysis)

  • NCoA-3 Antibody (M-397) is a rabbit polyclonal IgG provided at 200 µg/ml
  • epitope corresponding to amino acids 455-851 mapping within an internal region of NCoA-3 of mouse origin
  • recommended for detection of NCoA-3 and, to a lesser extent, GRIP-1 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, bovine and porcine
  • Available as TransCruz reagent for ChIP applications, sc-9119 X, 200 µg/0.1 ml
  • See 16 citations for NCoA-3 Antibody (M-397)
 

See additional NCoA Antibodies including NCoA, NCoA-3, NCoA-5 and NCoA-7.

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 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
NCoA-3 Antibody (M-397) sc-9119 200 µg/ml $279
NCoA-3 Antibody (M-397) X sc-9119 X 200 µg/0.1 ml $279
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
HeLa nuclear extract sc-2120 250 µg/0.05 ml $143
MIA PaCa-2 Cell Lysate sc-2285 500 µg/200 µl $104
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
Jurkat Whole Cell Lysate sc-2204 500 µg/200 µl $104
K-562 Whole Cell Lysate sc-2203 500 µg/200 µl $104
MCF7 Whole Cell Lysate sc-2206 500 µg/200 µl $104
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human NCOA3 8202 20q13.12 NM_006534, NM_181659 Q9Y6Q9
 601937
Mouse Ncoa3 17979 2 H3 O09000
 N/A
 

Nuclear receptors for steroids, thyroid hormones and retinoic acids are ligand-dependent transcription factors that activate transcription through specific DNA binding sites in their target genes. Several related transcriptional coactivators and corepressors have been described that work in concert with the steroid receptor family to either induce or repress transcription from hormone-responsive elements. This family includes GRIP1 (for GR interacting protein 1), also designated NCoA-2 or TIF-2; SRC-1 (for steroid receptor coactivator-1), also designated NCoA-1; and NCoA-3, also designated RAC3, ACTR, AIB-1 (for amplified in breast cancer) and p/CIP (for p300/CBP/co-integrator protein), which displays elevated expression in estrogen receptor positive ovarian and breast cancers and is required for the transcriptional activation of p300/CBP-dependent transcription factors.

NCoA-3 Antibody (M-397) Product Citations
See how others have used NCoA-3 Antibody (M-397) and or NCoA-3 Antibody (M-397) conjugates.


16 total citations
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NCoA-3 Antibody (M-397) Data
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sc-9119
NCoA-3 (M-397): sc-9119. Western blot analysis of His-tagged mouse recombinant NCoA-3.
sc-9119
NCoA-3 (M-397): sc-9119. Western blot analysis of NCoA-3 expression in K-562 whole cell lysate.
sc-9119
ChIP analysis of p52 promoter occupancy in murine p19 cells in response to activation by estradiol (E2). Antibodies tested include ERα (MC-20): sc-542, ERα (H-184): sc-7207, CBP (A-22): sc-369, CBP (C-1): sc-7300, p/CIP (F2): sc-5305, p/CIP (M-397): sc-9119, RIP140 (H-300): sc-8997 and HDAC3 (N-19): sc-11417. Data kindly provided by M.G. Rosenfeld and reproduced with permission from Perissi et al., Cell 2004, 116: 511-526.
sc-9119
ChIP analysis of cofactor occupancy dynamics on the ICAM1 promoter in 293 cells in response to IL-1β treatment. Antibodies tested include p300 (C-20): sc-sc-585, p300 (N-15): sc-584, p300 (H-272): sc-8981, NCoA-3 (F-2): sc-5305, NCoA-3 (M-397): sc-9119, NCoA-3 (N-17): sc-7217, NCoA-3 (C-20): sc-7216, SRC-1 (M-341): sc-8995, SRC-1 (C-20): sc-6096, SRC-1 (M-20): sc-6098 and GRIP1 (M-343): sc-8996. Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.
sc-9119
ChIP analysis of cofactor occupancy dynamics on the IL-8 promoter in 293 cells in response to IL-1β treatment. Antibodies tested include NCoA-3 (F-2): sc-5305, NCoA-3 (M-397): sc-9119, NCoA-3 (N-17): sc-7217, NCoA-3 (C-20): sc-7216, SRC-1 (M-341): sc-8995, SRC-1 (C-20): sc-6096, SRC-1 (M-20): sc-6098, GRIP1 (M-343): sc-8996, PCAF (C-16): sc-6300 and PCAF (H-369): sc-8999. Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.
sc-9119
ChIP analysis of in vivo binding of RORα and its recruitment of coactivators to RORα-responsive promoters in freshly dissected cerebella derived from wild type (+/+) and staggerer (Sg) mice. Control Input (A). Antibodies used included SRC-1 (M-341): sc-8995, SRC-1 (C-20): sc-6096 and SRC-1 (M-20): sc-6098 (B), Grip-1 (M-434): sc-8996 (C), NCoA-3 (F-2): sc-5305, NCoA-3 (M-397): sc-9119, NCoA-3 (N-17): sc-7217 and NCoA-3 (C-20): sc-7216 (D). Data kindly provided by M.G. Rosenfeld and reproduced wtih permission from Gold et al., Neuron 2003, 40: 1119-1131.
sc-9119
NCoA-3 (M-397): sc-9119. Immunofluorescence staining of normal mouse liver frozen section showing nuclear staining.
sc-9119
NCoA-3 (M-397): sc-9119. Western blot analysis of NCoA-3 expression in HeLa whole cell lysate.
sc-9119
NCoA-3 (M-397): sc-9119. Western blot analysis of NCoA-3 expression in HeLa whole cell lysate.
sc-9119
NCoA-3 (M-397): sc-9119. Western blot analysis of NCoA-3 expression in Jurkat (A) and K-562 (B) whole cell lysates.
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